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共軛
biotin conjugate
品質等級
形狀
lyophilized powder
分子量
~12 kDa
成份
Protein, ~40% Lowry
儲存溫度
2-8°C
SMILES 字串
CCOc1ccccc1C(=O)Nc2ccc(Cl)c(c2)C(F)(F)F
InChI
1S/C16H13ClF3NO2/c1-2-23-14-6-4-3-5-11(14)15(22)21-10-7-8-13(17)12(9-10)16(18,19)20/h3-9H,2H2,1H3,(H,21,22)
InChI 密鑰
YDXZSNHARVUYNM-UHFFFAOYSA-N
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一般說明
Cholera Toxin B (CTB) is secreted by Vibrio cholerae. CTB functions as an oral subunit vaccine for cholera, which is associated with acute watery diarrhoea. It acts as a mucosal immunogen. Cholera toxin (CT) stimulates cell surface molecules, such as antigen presenting cells (APCs), murine and human dendritic cells (DCs). CT also has immunomodulatory properties. It induces the secretion of interleukin 1 (IL-1) from macrophages and enhances their APC function.
應用
Cholera Toxin B subunit has been used:
- in immunofluorescence
- in the analysis of major histocompatibility complex (MHC) class II lipid raft partitioning
- in live cell three-dimensional tracking of SH-SY5Y human neuroblastoma cells
- to assess the toll-like receptors (TLR) and FcRγ (Fc receptor γ chain) – CARD9 (caspase recruitment domain family member 9) activation by cholera Toxin B (CTB)
生化／生理作用
The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.
品質
Biotin content ~1.0 mole/mole protein.
外觀
Lyophilized powder containing sodium phosphate buffer salts, sodium azide and sodium EDTA.
分析報告
Activity measured by ELISA using ganglioside GM1-coated multiwell plates, rabbit anti-Cholera toxin B subunit, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding is achieved with 0.02-1 μg of Cholera toxin B subunit-biotin conjugate per mL. The conjugated B subunit gives a similar value for 50% binding to that of unconjugated B subunit from which it is prepared.
危險聲明
防範說明
危險分類
Aquatic Chronic 3
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
3D tracking of single nanoparticles and quantum dots in living cells by out-of-focus imaging with diffraction pattern recognition
Gardini L, et al.
Scientific Reports, 5, 16088-16088 (2015)
Evidence for TLR4 and FcRgamma-CARD9 activation by cholera toxin B subunit and its direct bindings to TREM2 and LMIR5 receptors
Phongsisay V, et al.
Molecular Immunology, 66(2), 463-471 (2015)
The Ia. 2 epitope defines a subset of lipid raft-resident MHC class II molecules crucial to effective antigen presentation
Busman-Sahay K, et al.
Journal of Immunology, 1100336-1100336 (2011)
Expression of the native cholera toxin B subunit gene and assembly as functional oligomers in transgenic tobacco chloroplasts1
Daniell H, et al.
Journal of Molecular Biology, 311(5), 1001-1009 (2001)
Eva Koffeman et al.
Methods in molecular medicine, 136, 69-86 (2007-11-07)
T-cells specific for a particular antigen represent a small percentage of the overall T-cell population. Detecting the presence of antigen specific T-cells in patients, animal models or populations of cultured cells has presented a challenge to researchers. The T-cell capture
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