推荐产品
重組細胞
expressed in E. coli
形狀
lyophilized powder
比活性
≥50 units/mg protein
分子量
64 kDa
成份
Protein, ≥15% biuret
儲存溫度
−20°C
應用
胆固醇氧化酶已用于研究中以证明疏水 - 亲水嵌段共聚物系统中的微观异质性可用于固定酶和进行酶促反应。胆固醇氧化酶也被用于一项研究，该研究得出结论，激活胆固醇的两亲可能有助于治疗鼻咽癌。
胆固醇氧化酶用于测定血清胆固醇。该酶还可应用于食品样品中类固醇的微量分析以及区分 3-酮类固醇和 3β-羟基类固醇。表达胆固醇氧化酶的转基因植物正在与棉铃象甲作斗争中进行研究。CHOD 也被用作分子探针来阐明细胞膜结构。
生化／生理作用
胆固醇氧化酶 (CHOD) 是一种含有 FAD 的单体黄素蛋白，其催化胆固醇分解代谢的第一步。这种双功能酶在需要 FAD 的步骤中将胆固醇氧化为胆甾-5-烯-3-酮，然后随着H2O2的释放而异构化为胆甾-4-烯-3-酮。
物理性質
含 FAD 辅因子的 Ⅱ 型胆固醇氧化酶与该酶共价连接。
單位定義
在 pH 值为 7.5、25°C 条件下，每分钟 1 个单位的胆固醇将转化为 4-胆甾烯-3-酮 (1.0 μmol/min)。注：4-胆甾烯-3-酮可能发生异构化。
外觀
以含有蔗糖的冻干粉末形式提供
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
Block copolymer microdomains: a novel medium for enzymatic reactions
Gupte, A., et al.
Biotechnology Progress, 7, 348-354 (2012)
Meihe Zhang et al.
Biosensors & bioelectronics, 32(1), 288-292 (2011-12-31)
A novel cholesterol biosensor was prepared based on gold nanoparticles-catalyzed luminol electrogenerated chemiluminescence (ECL). Firstly, l-cysteine-reduced graphene oxide composites were modified on the surface of a glassy carbon electrode. Then, gold nanoparticles (AuNPs) were self-assembled on it. Subsequently, cholesterol oxidase
Laura Caldinelli et al.
The Journal of biological chemistry, 280(24), 22572-22581 (2005-04-09)
Cholesterol oxidase from Brevibacterium sterolicum is a monomeric flavoenzyme catalyzing the oxidation and isomerization of cholesterol to cholest-4-en-3-one. This protein is a class II cholesterol oxidases, with the FAD cofactor covalently linked to the enzyme through the His(69) residue. In
Effect of cholesterol concentration on organization of viral and vesicle membranes. Probed by accessibility to cholesterol oxidase.
R Pal et al.
The Journal of biological chemistry, 255(12), 5802-5806 (1980-06-25)
D R Corbin et al.
Plant physiology, 126(3), 1116-1128 (2001-07-18)
Cholesterol oxidase represents a novel type of insecticidal protein with potent activity against the cotton boll weevil (Anthonomus grandis grandis Boheman). We transformed tobacco (Nicotiana tabacum) plants with the cholesterol oxidase choM gene and expressed cytosolic and chloroplast-targeted versions of
实验方案
This procedure applies to products that have a specification for the enzymatic activity of cholesterol oxidase. This assay is NOT to be used to assay Cholesterol Oxidase from Schizophyllum commune (Discontinued Product Number C7274) and from Brevibacterium sp. (Discontinued Product Number C8153).
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门