推荐产品
生物源
rabbit
品質等級
共軛
unconjugated
抗體表格
IgG fraction of antiserum
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous solution
分子量
antigen 50 kDa
物種活性
human, chicken, rat, mouse
包裝
antibody small pack of 25 μL
技術
microarray: suitable
western blot: 1:5,000 using synaptosomal fraction of rat brain
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... CAMK2A(815)
mouse ... Camk2a(12322)
rat ... Camk2a(25400)
一般說明
Ca2+/Calmodulin dependent protein kinase II (CaMKII) belongs to the family of Ser/Thr protein kinases including CaMKI and CaMKIV. CaM kinase II is one of the most abundant protein kinases in the mammalian brain, with the highest expression in neurons of the hippocampus and the cerebral cortex. CaMKII consists of a family of four related isoforms CaMK IIα, CaMK IIβ, CaMK IIγ and CaMK IIδ. The CaMK II α and β isoforms are predominantly expressed in the brain, localized mainly in the cytosol and postsynaptic densities (PSDs), whereas the CaMKII γ and δ isoforms are expressed in all tissues. CaMKII contains a catalytic and regulatory domain. The regulatory domain consists of an autoinhibitory and calmodulin binding site.
特異性
The antibody does not cross-react with CaMKIIβ, γ and δ isoforms (60 kDa). Anti-CaM Kinase IIα specifically binds to CaM Kinase IIα (50kDa) in human, mouse, rat and chicken. CaM Kinase IIα immunizing peptide specifically inhibits the staining of CaM Kinase IIα in immunoblotting.
免疫原
Peptide corresponding to amino acids 461-478 of rat CaM Kinase Iiα, conjugated to KLH. This sequence is identical in human, mouse and chicken CaMKIIα has limited homology (50-60%) with CaM Kinase IIβ, γ and δ subunits.
應用
Anti-CaM Kinase IIα (CaMKIIα) antibody has been used:
- in immunolabelling
- in western blotting
- in immunoprecipitation
- in immunoblotting
生化/生理作用
CaMKII is involved in many cellular functions in response to Ca2+ signaling, including synthesis and secretion of neurotransmitters, axonal transport, long term potentiation (LTP) and spatial learning, receptor function and regulation of gene expression. CaMKII plays a critical role in LTP, a cellular model of learning and memory. In the CNS, postsynaptic Ca2+ influx triggers rapid autophosphorylation and stable activation of CaMKII in a Ca2+/calmodulin dependent manner at a threonine residue in the autoinhibitory domain. Autophosphorylation of CaMKIIα at Thr286 has been shown to be required for LTP and learning.
外觀
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
nwg
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
The Journal of biological chemistry, 291(52), 26636-26646 (2016-11-07)
Calmodulin (CaM) is a Ca
Nature communications, 7, 12885-12885 (2016-10-05)
Molecularly defined subclassification is associated with phenotypic malignancy of glioblastoma (GBM). However, current understanding of the molecular basis of subclass conversion that is often involved in GBM recurrence remain rudimentary at best. Here we report that canonical Wnt signalling that
Structure of the autoinhibited kinase domain of CaMKII and SAXS analysis of the holoenzyme
Cell, 123(5), 849-860 (2005)
CaMKII beta functions as an F-actin targeting module that localizes CaMKII alpha beta heterooligomers to dendritic spines
Neuron, 21(3), 593-606 (1998)
Communicative & integrative biology, 2(1), 40-41 (2009-08-26)
CaMKII, calcium/calmodulin dependent protein kinase, is an active kinase in the cell that phosphorylates a number of substrates including several cytoskeletal and signaling proteins. In addition to kinase activity, the beta isoform of CaMKII also contains an F-actin binding region.
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