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一般說明
Baseclick EdU HTS Kit为直接检测DNA合成提供了替代BrdU和[3H]thymidine检测的出色方法。EdU（5-乙炔基-2'.-脱氧尿苷）是一种胸苷核苷类似物，在活性DNA合成期间掺入到DNA之中。与BrdU检测法不同，EdU HTS Assay不依赖抗体，因而不需进行DNA变性来检测掺入的核苷。恰恰相反，EdU HTS Kit利用点击化学法来检测不同种类的染料荧光示值。此外，该试剂盒的实验方案经过简化，减少了总步骤数同时大幅缩减了总耗时。简单的点击化学法检测步骤可在30分钟内完成，在结果的内容和情境丰富程度方面，堪比多重分析方法。
應用
高通量筛选
訊號詞
Danger
危險分類
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Muta. 1B - Repr. 2 - Skin Corr. 1C
安全危害
儲存類別代碼
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Yongmao Yu et al.
Journal of immunological methods, 350(1-2), 29-35 (2009-08-04)
T lymphocyte proliferations can be measured by [(3)H]thymidine incorporation. However, many labs avoid this technique because of the need to use radioactive substrates. In addition, [(3)H]thymidine incorporation method does not permit simultaneous characterization of the proliferating cells. We developed the
Fatemah Chehrehasa et al.
Journal of neuroscience methods, 177(1), 122-130 (2008-11-11)
Labelling and identifying proliferating cells is central to understanding neurogenesis and neural lineages in vivo and in vitro. We present here a novel thymidine analogue, ethynyl deoxyuridine (EdU) for labelling dividing cells, detected with a fluorescent azide which forms a
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