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Merck
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文件

B6777

Sigma-Aldrich

5-溴-4-氯-3-吲哚磷酸 对甲苯胺盐

BioReagent, ≥99%, powder, suitable for molecular biology

别名:

BCIP® 对甲苯胺盐, X-磷酸盐 对甲苯胺盐

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About This Item

经验公式（希尔记法）:
C8H6BrClNO4P · C7H9N
CAS号:
6578-06-9
分子量:
433.62
EC號碼:
229-506-1
MDL號碼:
MFCD00040636
分類程式碼代碼:
12352204
PubChem物質ID:
24891823
NACRES:
NA.83

product name

5-溴-4-氯-3-吲哚磷酸 对甲苯胺盐, BioReagent, for molecular biology, powder, ≥99%

等級

for molecular biology

產品線

BioReagent

化驗

≥99%

形狀

powder

溶解度

DMF: 20 mg/mL
H2O: insoluble

異物活動

Protease, none detected

儲存溫度

−20°C

SMILES 字串

Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23

InChI

1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3

InChI 密鑰

QEIFSLUFHRCVQL-UHFFFAOYSA-N

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應用

用于碱性磷酸酶标记分子的比色检测，可使用5-溴-4-氯-3-吲哚基磷酸酯（BCIP）和硝基蓝四唑（NBT）。BCIP/NBT底物系统具有多种功能并用于多种应用，包括Northern Southern和Western blotting、原位杂交和免疫组化。
BCIP以两种盐形式提供：可溶于水的二钠盐和可溶于二甲基甲酰胺的对甲苯胺形式。这些盐形式可用于制备与NBT及反应缓冲液组合的储存溶液，并形成碱性磷酸酶的底物溶液。当与碱性磷酸酶一起孵育时，该底物系统可产生不溶的NBT二甲嗪产物，具有容易被观察到的紫色。为了方便起见，BCIP和NBT的混合物是以一种易溶解的片剂形式或作为即用型液体而提供的。

包裝

Bottomless glass bottle. Contents are inside inserted fused cone.

基底

碱性磷酸酶的组织化学底物。

法律資訊

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

個人防護裝備

dust mask type N95 (US), Eyeshields, Gloves

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