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B6650

Sigma-Aldrich

X-GlcA

reagent for selection of recombinant bacterial clones

别名:

5-溴-4-氯-3-吲哚基 β-D-葡糖醛酸 环己铵盐, X-GlcA, X-葡糖苷酸 CHA 盐

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About This Item

经验公式（希尔记法）:
C14H13BrClNO7 · C6H13N
CAS号:
114162-64-0
分子量:
521.79
MDL號碼:
MFCD00058543
分類程式碼代碼:
12352200
PubChem物質ID:
24891926
NACRES:
NA.85

等級

Molecular Biology Reagent
for molecular biology

品質等級

200

無菌

non-sterile

化驗

≥98% (TLC)

形狀

powder

技術

nucleic acid detection: suitable

溶解度

DMF: soluble

適合性

suitable for β-galactosidase test

儲存溫度

−20°C

SMILES 字串

NC1CCCCC1.O[C@@H]2[C@@H](O)[C@@H](O[C@@H]([C@H]2O)C(O)=O)Oc3c[nH]c4ccc(Br)c(Cl)c34

InChI

1S/C14H13BrClNO7.C6H13N/c15-4-1-2-5-7(8(4)16)6(3-17-5)23-14-11(20)9(18)10(19)12(24-14)13(21)22;7-6-4-2-1-3-5-6/h1-3,9-12,14,17-20H,(H,21,22);6H,1-5,7H2/t9-,10-,11+,12-,14+;/m0./s1

InChI 密鑰

JXCKZXHCJOVIAV-CYRSAHDMSA-N

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一般說明

X-GlcA 是 β-半乳糖苷酶的显色底物，用于确定琼脂平板上生长的细菌中是否存在克隆的 DNA 插入片段。 X-GlcA 设计用于具有 lac+ 表型的重组细菌菌落的蓝白色选择中替代 X-Gal。

應用

适用于选择具有 lac+ 表型的重组细菌菌落。
X-GlcA 已用于根切片的组化染色，用于光学显微镜观察。
β-葡萄糖醛酸酶 (GUS) 基因检测的显色底物。

原則

当 X-GlcA 被 β-半乳糖苷酶水解时，所得产物将形成蓝色沉淀。在 X-GlcA 存在下生长的 Lac+ 菌落会变成深蓝色，从而易于区分 lac+ 和 lac- 菌落。

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