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Merck

B5388

Sigma-Aldrich

亮甲酚蓝 ALD

certified by the Biological Stain Commission

别名:

3-氨基-7-(二乙氨基)-2-甲基吩恶嗪-5-铵 (T-4)-四氯锌酸盐 (2:1), 亮甲酚蓝, 亮蓝 C

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About This Item

线性分子式:
C17H20N3OCl · 1/2ZnCl2
CAS号:
分子量:
385.96
顏色索引編號:
51010
Beilstein:
5690713
EC號碼:
MDL號碼:
分類程式碼代碼:
12171500
PubChem物質ID:
NACRES:
NA.47

等級

certified by the Biological Stain Commission

品質等級

形狀

powder

成份

Dye content, ≥60%

溶解度

water: 1 mg/mL, blue to very deep blue

應用

diagnostic assay manufacturing
hematology
histology

儲存溫度

room temp

InChI

1S/2C17H20N3O.4ClH.Zn/c2*1-4-20(5-2)12-6-7-14-16(9-12)21-17-10-13(18)11(3)8-15(17)19-14;;;;;/h2*6-10H,4-5,18H2,1-3H3;4*1H;/q2*+1;;;;;+2/p-4

InChI 密鑰

SIHASHFMDFXGSX-UHFFFAOYSA-J

應用

灿烂甲酚蓝 ALD 已用于卵母细胞的选择。它也被用于选择卵丘-卵母细胞复合体 (COCs)。

生化/生理作用

灿烂甲酚蓝 ALD 主要用于卵母细胞的选择。它有助于测定葡萄糖-6-磷酸脱氢酶 (G6PD) 的活性。这种酶在生长的卵母细胞中产生。然而,完成生长的卵母细胞 G6PD 水平较低。由于亮甲酚蓝不能还原成无色化合物,所以低 G6PD 活性导致细胞质呈蓝色。它也用于网织红细胞染色,其中染料染色网硬蛋白。

適合性

经认证为网织红细胞的活上染色。

其他說明

灿烂甲酚蓝 ALD 的结构在吩恶嗪核心上含有 2-甲基修饰,而历史性灿烂甲酚蓝的结构在吩恶嗪核心上含有 8-甲基修饰。两种结构的生物染色应用相同。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Gloves


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