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生物源
goat
共軛
alkaline phosphatase conjugate
抗體表格
affinity isolated antibody
抗體產品種類
secondary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous solution
技術
direct ELISA: 1:50,000
dot blot: 1:60,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
western blot (chemiluminescent): 1:60,000
運輸包裝
wet ice
儲存溫度
2-8°C
目標翻譯後修改
unmodified
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一般說明
There are five immunoglobulin classes in humans. Out of these, immunoglobulin M (IgM ) is a high molecular weight protein that has five or six subunits. IgM monomers are made of two heavy chains and two light chains connected by a disulfide bond. Human serum shows low concentration of IgM monomers. It has a high carbohydrate content of about 12%. It is the first immunoglobulin produced by neonates.
特異性
Goat polyclonal anti-Human IgM (μ-chain specific)-Alkaline Phosphatase antibody is specific for human IgM when tested against human IgA, IgG, IgM, Bence Jones kappa, and lambda myeloma proteins. The conjugate shows no reactivity with mouse or rat IgG.
應用
IgM是一种糖蛋白，重链上具有5个n连接的糖基化位点。可通过ELISA分析确定结合凝集素的IgM的糖基化形式，其中碱性磷酸酶标记的山羊抗人IgM可作为二抗（1:2000稀释），以对硝基苯基为底物(Sigma)。
生化／生理作用
Immunoglobulin M (IgM) acts as an antigen specific part of the B cell antigen receptor on the surface of B lymphocytes that are not stimulated, in its monomeric form. Polymeric IgM molecules also serve as important activators of the classical complement cascade. IgM is essential in agglutination and cytolytic reactions.
Immunoglobulin M (IgM) antibodies appear early in the course of infections. IgM antibodies are responsible for agglutination of red blood cells in mis-matched blood transfusions. The level of IgM may vary with the status of disease or infection. Alkaline Phosphatase is an enzyme that catalyzes the conversion of chromogenic substrates such as p-nitrophenylphosphate (PNPP); chemiluminescent substrates such as CDP-Star® and fluorogenic substrates such as 4-methylumbelliferyl phosphate (4-MUP) into detectable chromophores, light-emitters or fluorescers, respectively.
其他說明
No cross-reaction with mouse and rat IgG
外觀
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide
法律資訊
CDP-Star is a registered trademark of Tropix, Inc.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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