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Merck

A6103

Sigma-Aldrich

Aprotinin

recombinant, expressed in Nicotiana (tobacco), ≥5 TIU/mg protein, powder, ≥98% (SDS-PAGE), suitable for inhibition assay

别名:

BPTI, Basic pancreatic trypsin inhibitor, Kallikrein-trypsin inactivator, Kunitz protease inhibitor

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About This Item

EC號碼:
分類程式碼代碼:
12352202
NACRES:
NA.77

product name

Aprotinin Nicotiana tobacco, >= 5TIU/mg protein, >= 98 % SDS-PAGE | 9087-70-1, recombinant, expressed in Nicotiana (tobacco), ≥5 TIU/mg protein, ≥98% (SDS-PAGE)

重組細胞

expressed in Nicotiana (tobacco)

品質等級

描述

recombinant form of thenative bovine-sequence aprotinin purified from plant tissue (tobacco)

產品線

BioUltra

化驗

≥98% (SDS-PAGE)

形狀

powder

比活性

≥5 TIU/mg protein

分子量

~_6.5 kDa

技術

inhibition assay: suitable

溶解度

0.85% sodium chloride: 5 mg/mL

UniProt登錄號

儲存溫度

2-8°C

基因資訊

cow ... PTI(404172)

單位定義

一个胰蛋白酶抑制剂单位 (TIU) 将使 2 个胰蛋白酶单位的活性下降 50%,其中 1 个胰蛋白酶单位将在 25°C pH 7.8 下每分钟水解 1.0 μmol 的 N-α-苯甲酰-DL-精氨酸对硝基苯胺 (BAPNA)。

準備報告

不含动物源性成分或杂质,通过在非转基因烟草属植物中繁殖的 RNA (+) 链烟草花叶病毒载体中瞬时表达抑肽酶信息生产。抑肽酶是天然的牛抑肽酶的重组形式,传统上通过分级沉淀、凝胶过滤和离子交换色谱法从牛肺中分离得到。与动物来源的抑肽酶不同,本品采用专有方法从植物组织中分离纯化。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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实验方案

Objective: To standardize a procedure for the enzymatic assay of Aprotinin.

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