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生物源
mouse
共軛
alkaline phosphatase conjugate
抗體表格
purified from hybridoma cell culture
抗體產品種類
primary antibodies
無性繁殖
1A4, monoclonal
形狀
buffered aqueous glycerol solution
分子量
antigen ~42 kDa
物種活性
human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake
技術
ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20 using human tonsil or appendix sections
western blot: 1:100 using chicken gizzard extract/ Mouse heart extract
同型
IgG2a
UniProt登錄號
運輸包裝
wet ice
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
mouse ... Acta2(11475)
rat ... Acta2(81633)
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一般說明
其抗体(也称为抗α-Sm-1)对α-平滑肌肌动蛋白的单一异构体具有特异性。它在免疫印迹试验中可与α-平滑肌肌动蛋白发生特异性反应,并可在冷冻或福尔马林固定的、或石蜡包埋的组织切片中对平滑肌细胞进行标记。
肌动蛋白是一种高度保守的蛋白质,是所有细胞类型中细胞骨架和收缩结构的主要组成部分。肌动蛋白含量与分化类型以及细胞和组织的功能状态有关。肌动蛋白具有两种不同的聚集形式,即球状或纤维状,并且在脊椎动物中至少具有六种不同亚型。肌动蛋白表现出大于90%的序列同源性,但每种亚型都具有不同的NH 2-末端序列。同工型由三种α肌动蛋白(骨骼肌,心脏,平滑肌),一种β肌动蛋白(β-非肌肉)和两种γ肌动蛋白(γ平滑肌和γ非肌肉)组成。
免疫原
N-末端合成的α-平滑肌肌动蛋白十肽。
應用
Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.
使用单克隆抗ACTA2抗体对来自牛主动脉的平滑肌细胞进行免疫细胞化学分析。首先将细胞在玻璃盖玻片上生长,并在4%的50%丙酮/乙醇中固定10分钟。
对固定在1%PFA中的角质细胞进行免疫细胞化学,并与小鼠单克隆抗平滑肌肌动蛋白(14A)以1:100的稀释度孵育。
小鼠单克隆抗肌动蛋白,α−平滑肌-碱性磷酸酶抗体可用于小鼠和人体组织的免疫组织化学应用。
成功使用该抗体的应用以及相关的同行评审论文如下所示。
免疫组织化学(1篇论文)
免疫组织化学(1篇论文)
外觀
含有1 mM MgCl2、1%牛血清白蛋白、50%甘油和15 mM叠氮化钠(用作防腐剂)的0.05M Tris缓冲液,pH 8.0中的溶液。
其他說明
想要查看肌动蛋白抗体选择指南,请访问 www.sigmaaldrich.com/actin。
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 2
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
其他客户在看
The American journal of pathology, 175(1), 148-160 (2009-06-23)
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