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生物源
bovine
品質等級
化驗
≥98% (agarose gel electrophoresis)
形狀
lyophilized powder
分子量
~66 kDa
純化經由
heat shock fractionation
包裝
poly bottle of
來源
USA origin
技術
ELISA: suitable
雜質
essentially globulin free
≤1 ng/mg endotoxin
pH值
5.2
溶解度
water: soluble (40 mg/ml)
UniProt登錄號
異物活動
BT Virus, none detected
VSV Virus, none detected
儲存溫度
2-8°C
基因資訊
bovine ... ALB(280717)
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一般說明
Bovine Serum Albumin (BSA) is a globular non-glycosylated protein that encompasses structurally distinct domains (I, II, and III) with two sub-domains, A and B within each domain. Structurally, BSA is an α-helical protein with 17 cysteine residues.
應用
Bovine Serum Albumin has been used:
- as a component of the minimal liquid medium to culture Porphyromonas gingivalis strains
- as a standard protein to quantify microsomes homogenate using Bradford method
- as a blocking agent in enzyme-linked immunosorbent assay (ELISA) of fecal lactoferrin
生化／生理作用
BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。
Bovine Serum Albumin (BSA) is widely used in drugs and antibody delivery. It also finds applications in nanoparticle preparations and is useful in targeting tumor tissues. BSA is non-immunogenic and non-toxic. It also shows binding with metal ions.
特點和優勢
- Low endotoxin
- Essentially globulin-free
準備報告
血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此，有些人便开始使用“Fraction V”这一术语来描述血清白蛋白，无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白，而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白，包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
采用热休克分级分离法制备
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
Keitarou Saiki et al.
Journal of oral biosciences, 62(4), 315-321 (2020-09-17)
Porphyromonas gingivalis is one of the etiologic agents of chronic periodontitis. Our previous study showed that the use of minimal media for P. gingivalis allowed to isolate novel inhibitors of P. gingivalis growth. However, growth of P. gingivalis in minimal media was not
Nancy Machorro-Rojas et al.
Molecular medicine reports, 20(3), 2083-2090 (2019-07-02)
The intestinal epithelium is a monolayer of cells arranged side‑by‑side and connected by tight junction (TJ) proteins expressed at the apical extreme of the paracellular membrane. This layer prevents stress‑induced inflammatory responses, thus helping to maintain gut barrier function and
Tamara Topală et al.
Clujul medical (1957), 87(4), 215-219 (2014-01-01)
The continuous search for new molecules with therapeutic abilities has led to the synthesis and characterization of a large number of metal complexes, proven to exhibit potential as pharmacological agents through their antibacterial, antiviral, antifungal and antineoplastic properties. As serum
Balázs Rada et al.
Journal of immunology (Baltimore, Md. : 1950), 181(7), 4883-4893 (2008-09-20)
The dual oxidase-thiocyanate-lactoperoxidase (Duox/SCN(-)/LPO) system generates the microbicidal oxidant hypothiocyanite in the airway surface liquid by using LPO, thiocyanate, and Duox-derived hydrogen peroxide released from the apical surface of the airway epithelium. This system is effective against several microorganisms that
Ignacio Izeddin et al.
PloS one, 6(1), e15611-e15611 (2011-01-26)
The actin cytoskeleton of dendritic spines plays a key role in morphological aspects of synaptic plasticity. The detailed analysis of the spine structure and dynamics in live neurons, however, has been hampered by the diffraction-limited resolution of conventional fluorescence microscopy.
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