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Merck

A1806

Sigma-Aldrich

单克隆抗-磷酸酪氨酸

clone PT-66, purified immunoglobulin, PBS solution

别名:

Monoclonal Anti-Phosphotyrosine, Phospho-Tyr, Phospho-tyrosine, p-Tyr

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.44

生物源

mouse

品質等級

重組細胞

expressed in mouse cell line

共軛

agarose conjugate

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

PT-66, monoclonal

形狀

PBS solution

技術

immunoprecipitation (IP): suitable

同型

IgG1

容量

1 mg/mL binding capacity

運輸包裝

wet ice

儲存溫度

2-8°C

目標翻譯後修改

phosphorylation (pTyr)

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相关类别

一般說明

经 ELISA 和竞争性 ELISA 确定,该抗体与磷酸化的酪氨酸特异性反应,该磷酸化的酪氨酸既可以是游离氨基酸形式,也可与 BSA 或 KLH 等载体结合的形式。与非磷酸化的酪氨酸、磷酸苏氨酸、磷酸丝氨酸、AMP 或 ATP 没有观察到交叉反应。
Monoclonal Anti-Phosphotyrosine (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

免疫原

与 BSA 结合的磷酸酪氨酸

應用

Monoclonal Anti-Phosphotyrosine-Agarose antibody produced in mouse has been used in:
  • immunoprecipitation experiments for affinity-purification of phosphotyrosine proteins
  • phosphotyrosine pulldown assays
  • PI3-kinase assay

Proteins containing phosphotyrosines were deteced in protein extracts from dissected tissue of Harlan Sprague Dawley rats or from transfected HEK293 cells using mouse monoclonal anti-phosphotyrosine antibody as the primary antibody. Reactivity was blocked when extracts were previously treated with 10 mM phosphotyrosine but not 10 mM tyrosine showing the specifity of the antibody for phosphorylated tyrosines. Immunoprecipitation of proteins containing phosphorylated tyrosines in rat dorsat root ganglion homogenates was performed using mouse monoclonal anti-phosphotyrosine. The antibody was incubated with the homogenates in the presence of 50 mm NaF and protease inhibitors for 4-14 hours at 4°. The antibody was precipitated using Protein G-agarose beads incubated overnight.

生化/生理作用

Reversible phosphorylation of proteins is an important post-translational modification that plays a regulatory role in the expression of most proteins in the cells. Reversible phosphorylation at multiple serine, tyrosine and threonine residues mediate numerous signalling pathways in both prokaryotic and Eukaryotic cells. Cellular proteins with phosphorylated tyrosine increase many fold by the activation of tyrosine kinases. Most mitogenic receptor systems such as EGF, PDGF, insulin receptors contain tyrosine kinase domains that undergo autophosphorylation when receptors bind to the respective ligands. Tyrosine-specific protein kinase activity has also been described in many retroviral oncogene proteins. Cells transformed by these oncogenes contain elevated levels of phosphotyrosine. Many of the oncogenes found in mammalian oncogenic viruses encode tyrosine protein kinases that reside in the cellular cytoplasm. Others encode transmembrane receptors whose tyrosine phosphotransferase activity is stimulated by the binding of ligand to the extracellular domain.

外觀

Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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J A Houmard et al.
The American journal of physiology, 277(6 Pt 1), E1055-E1060 (1999-12-22)
The purpose of this study was to determine if the improvement in insulin sensitivity with exercise training is associated with enhanced phosphatidylinositol 3-kinase (PI 3-kinase) activity. Nine sedentary men were studied before and after 7 days of exercise training (1
M S Hickey et al.
Journal of applied physiology (Bethesda, Md. : 1985), 83(3), 718-722 (1997-09-18)
The purpose of this investigation was to determine whether insulin-stimulated phosphatidylinositol 3-kinase (PI3-kinase) activity is detectable in needle biopsies of human skeletal muscle. Sixteen healthy nonobese males matched for age, percent fat, fasting insulin, and fasting glucose participated in one
Insulin activation of phosphatidylinositol 3-kinase in human skeletal muscle in vivo
Hickey MS, et al.
Journal of Applied Physiology, 83(3), 718-722 (1997)
Tyrosine 116 of the herpes simplex virus type 1 IE alpha 22 protein is an ocular virulence determinant and potential phosphorylation site
Brandt CR and Kolb AW
Investigative Ophthalmology & Visual Science, 44(11), 4601-4607 (2003)
RET oncoproteins induce tyrosine phosphorylation changes of proteins involved in RNA metabolism
Gorla L, et al.
Cellular Signalling, 18(12), 2272-2282 (2006)

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