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Merck

A1233

Sigma-Aldrich

AMPK (α1/β1/γ1),活性,His标记 人

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

别名:

AMPKG, HAMPKb, MGC17785, MGC33776, MGC57364, MGC8666, PRKAA1, PRKAB1, PRKAG1

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.32

生物源

human

品質等級

重組細胞

expressed in baculovirus infected Sf9 cells

產品線

PRECISIO® Kinase

化驗

≥70% (SDS-PAGE)

形狀

buffered aqueous glycerol solution

比活性

626-848 nmol/min·mg

分子量

A1 subunit ~68 kDa
B1 subunit ~38 kDa
G1 subunit ~40 kDa

溶解度

water: soluble

UniProt登錄號

應用

life science and biopharma

運輸包裝

dry ice

儲存溫度

−70°C

基因資訊

一般說明

研究领域:细胞信号传导



AMP活化蛋白激酶(AMPK)是一种异源三聚体蛋白激酶,由α催化亚基、非催化β亚基和γ亚基组成。

應用

AMP活化蛋白激酶(AMPK)可用于通过生物层干涉(Bio-Layer Interferometry,BLI)结合测定,评估芬氏唐松草碱(thalidezine)与重组活性人 AMPK的结合动力学。(1)

生化/生理作用

AMP活化蛋白激酶(AMPK)是一种重要的能量感应酶(energy-sensing enzyme),可监测细胞能量状态。响应细胞代谢应激,AMPK被激活并磷酸化和灭活乙酰辅酶A羧化酶(ACC)和3-羟基-3-甲基戊二酰辅酶A还原酶(HMGCR),这是调节脂肪酸和胆固醇生物合成的关键酶。

外觀

50 mM 磷酸氢二钠 pH 7.0、300 mm NaCl、150 mm 咪唑、0.1 mM PMSF、0.2 mm DTT、25% 甘油中提供。

法律資訊

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

象形圖

Health hazardExclamation mark

訊號詞

Danger

危險聲明

危險分類

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


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Yasuhiko Minokoshi et al.
Nature, 428(6982), 569-574 (2004-04-03)
Obesity is an epidemic in Western society, and causes rapidly accelerating rates of type 2 diabetes and cardiovascular disease. The evolutionarily conserved serine/threonine kinase, AMP-activated protein kinase (AMPK), functions as a 'fuel gauge' to monitor cellular energy status. We investigated
D G Hardie et al.
European journal of biochemistry, 246(2), 259-273 (1997-06-01)
A single entity, the AMP-activated protein kinase (AMPK), phosphorylates and regulates in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase (key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively), and probably many additional targets. The kinase is activated by high

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We present an article about how proliferating cells require the biosynthesis of structural components for biomass production and for genomic replication.

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