生物源
rabbit
品質等級
共軛
unconjugated
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
物種活性
rat, mouse
技術
immunohistochemistry: suitable using Mouse kidney sections
western blot: 1:200 using Rat kidney membranes
UniProt登錄號
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
mouse ... Aqp3(11828)
rat ... Aqp3(65133)
一般說明
Aquaporins function in the movement of water and small solutes across the cell membrane. Aquaporins are widely distributed from bacteria to plants and animals. In E. coli, one aquaporin has been identified that is named AQP-Z. In the plant Arabidopsis thaliana, over 9 AQPs have been characterized. In mammals, at least 11 different AQPs have been cloned. AQP3 provides kidney medullary collecting ducts with high permeability to water, permitting water to move in the direction of an osmotic gradient and may play an important role in gastrointestinal tract water transport. AQP3 and AQP4 are located at the basolateral part of epithelial cells in kidney, colon and trachea.
Rabbit polyclonal anti-Water Channel Aquaporin 3 recognizes rat and mouse aquaporin 3 transport proteins.
免疫原
synthetic peptide corresponding to amino acids 275-292 of rat aquaporin 3 (with additional N-terminal lysine and tyrosine) conjugated to KLH with glutaraldehyde
應用
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunofluorescence (1 paper)
Rabbit anti-AQP3 antibody was used in western blot analysis to identify the level of aquaporin 3 in E. solidaginis larvae. Anti-AQP3 was diluted 1:200 in 5% nonfat dry milk and incubated with the membrane for 2hrs at 21 degrees C.
Rabbit polyclonal anti-Water Channel Aquaporin 3 is used to tag aquaporin 3 for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of aquaporin 3 in kidney medullary collecting ducts and the basolateral surfaces of epithelial cells involved in water transport.
外觀
Lyophilized from a solution containing phosphate buffered saline, pH 7.4, 1% BSA, and 0.05% sodium azide
準備報告
Affinity isolated on immobilized Protein A
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
K Ishibashi et al.
The American journal of physiology, 272(2 Pt 2), F235-F241 (1997-02-01)
Aquaporin-3 (AQP3) is unique in its structure (lowest homology with other aquaporins) and in its function (significantly conductive to both small nonelectrolytes and water). However, there is a controversy among researchers on its water transport and induction by dehydration. We
C A Ecelbarger et al.
The American journal of physiology, 269(5 Pt 2), F663-F672 (1995-11-01)
The aquaporins are a family of water channels expressed in several water-transporting tissues, including the kidney. We have used a peptide-derived, affinity-purified polyclonal antibody to aquaporin-3 (AQP-3) to investigate its localization and regulation in the kidney. Immunoblotting experiments showed expression
Susumu Yoshie et al.
Journal of cellular physiology (2019-02-05)
Functional central airway epithelial cells (CAECs) from induced pluripotent stem cells (iPSCs) are an attractive potential cell source for central airway regeneration. The central airway epithelium, such as the tracheal epithelium, is composed of ciliated cells, goblet cells, and basal
Susumu Yoshie et al.
Journal of cellular physiology, 234(9), 15951-15962 (2019-02-05)
Functional central airway epithelial cells (CAECs) from induced pluripotent stem cells (iPSCs) are an attractive potential cell source for central airway regeneration. The central airway epithelium, such as the tracheal epithelium, is composed of ciliated cells, goblet cells, and basal
M Echevarria et al.
Proceedings of the National Academy of Sciences of the United States of America, 91(23), 10997-11001 (1994-11-08)
The terminal part of the inner medullary collecting duct exhibits a high degree of water permeability that is independent of increased intracellular cAMP and not accounted for by the activity of the known renal epithelial water channels CHIP28 (28-kDa channel-forming
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