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96669

Sigma-Aldrich

7-二乙氨基-3-（4-马来酰亚胺苯基）-4-甲基香豆素

suitable for fluorescence, BioReagent, ≥90% (HPCE)

别名:

1 H -吡咯-2,5-二酮，1-（4-（7-（二乙氨基）-4-甲基-2-氧代-2 H -1-苯并吡喃-3-基）苯基）-, CPM

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About This Item

经验公式（希尔记法）:
C24H22N2O4
CAS号:
76877-33-3
分子量:
402.44
MDL號碼:
MFCD00077334
分類程式碼代碼:
12171500
PubChem物質ID:
57653774
NACRES:
NA.32

產品線

BioReagent

品質等級

100

化驗

≥90% (HPCE)

形狀

solid

螢光

λex 391 nm; λem 472 nm in methanol: 2-mercaptoethanol

適合性

suitable for fluorescence

儲存溫度

−20°C

SMILES 字串

CCN(CC)c1ccc2C(C)=C(C(=O)Oc2c1)c3ccc(cc3)N4C(=O)C=CC4=O

InChI

1S/C24H22N2O4/c1-4-25(5-2)18-10-11-19-15(3)23(24(29)30-20(19)14-18)16-6-8-17(9-7-16)26-21(27)12-13-22(26)28/h6-14H,4-5H2,1-3H3

InChI 密鑰

YGIABALXNBVHBX-UHFFFAOYSA-N

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應用

完整细胞的谷胱甘肽荧光探针；具体优点：只有硫代衍生物荧光，高量子产率和良好的斯托克斯位移

生化／生理作用

用于监测硫醇的释放，定量微孔板反应中的硫醇，并通过核仁蛋白染色区分增殖的癌细胞。

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

分析证书(COA)

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D J Steenkamp
The Biochemical journal, 292 ( Pt 1), 295-301 (1993-05-15)
Methods for the qualitative and quantitative analysis of thiols by means of the fluorogenic reagent 7-diethylamino-3-(4'-maleimidylphenyl)-4-methylcoumarin are described, with particular reference to the trypanosomatid metabolites glutathionylspermidine (GSH-spermidine) and trypanothione. Second-order rate constants for the derivatization of seven different thiols under
W Feng et al.
The Journal of biological chemistry, 275(46), 35902-35907 (2000-09-22)
Inositol 1,4,5-trisphosphate receptors (IP(3)R) and ryanodine receptors (RyR) mediate the release of endoplasmic and sarcoplasmic reticulum (ER/SR) Ca(2+) stores and regulate Ca(2+) entry through voltage-dependent or ligand-gated channels of the plasma membrane. A prominent property of ER/SR Ca(2+) channels is
F C Ayers et al.
Analytical biochemistry, 154(1), 186-193 (1986-04-01)
A microfluorometric assay for thiols has been developed using the thiol-specific fluorochrome N-[4-(7-diethylamino-4-methyl-3-coumarinyl)phenyl]maleimide (CPM). The technique may be used to quantitate either cellular or plasma thiols over a range of 0.01 to 3.0 nmol and may be used with as
Victor Goncalves et al.
Analytical biochemistry, 421(1), 342-344 (2011-11-05)
N-myristoylation is the irreversible attachment of a C(14) fatty acid, myristic acid, to the N-terminal glycine of a protein via formation of an amide bond. This modification is catalyzed by myristoyl-coenzyme A (CoA):protein N-myristoyltransferase (NMT), an enzyme ubiquitous in eukaryotes
Alice Talpin et al.
Arthritis research & therapy, 16(4), 417-417 (2014-08-22)
This study aimed to compare the functional capacity and gene expression profile of monocyte-derived dendritic cells (MD-DCs) in HLA-B27+ axial spondyloarthritis (SpA) patients and healthy controls. MD-DCs were differentiated with interleukin 4 (IL-4) and granulocyte-macrophage colony-stimulating factor (GM-CSF) for seven
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