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Merck

94403

Sigma-Aldrich

Hoechst 33258 溶液

1 mg/mL in H2O, ≥98.0% (HPLC)

别名:

双苯并咪唑H 33258溶液, 赫斯特荧光染料 33258 溶液

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About This Item

经验公式(希尔记法):
C25H24N6O · 3HCl
CAS号:
分子量:
533.88
分類程式碼代碼:
12171500
PubChem物質ID:
NACRES:
NA.32

品質等級

化驗

≥98.0% (HPLC)

形狀

liquid

濃度

1 mg/mL in H2O

溶解度

DMF: soluble
H2O: soluble

螢光

λex 355 nm; λem 465 nm in TE buffer; DNA

儲存溫度

2-8°C

SMILES 字串

Cl.Cl.Cl.CN1CCN(CC1)c2ccc3[nH]c(nc3c2)-c4ccc5nc([nH]c5c4)-c6ccc(O)cc6

InChI

1S/C25H24N6O.3ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16;;;/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29);3*1H

InChI 密鑰

SMNPLAKEGAEPJD-UHFFFAOYSA-N

一般說明

赫斯特 33258,也被称为双联苯亚胺,是一种蓝色荧光染料。它被归类为一种染色体染剂,可与DNA双螺旋的小沟结合,识别一组四对A-T碱基对。

應用

赫斯特 33258被用于荧光法估算样品中DNA浓度,这种方法比分光光度法更好,它可以测定纳克级DNA。并且,由于赫斯特 33258适用于识别细胞培养基中的支原体污染,由于其高荧光性。

儲存類別代碼

10 - Combustible liquids

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Cornelia Fritsch et al.
PLoS genetics, 15(7), e1008269-e1008269 (2019-07-13)
Development of eye tissue is initiated by a conserved set of transcription factors termed retinal determination network (RDN). In the fruit fly Drosophila melanogaster, the zinc-finger transcription factor Glass acts directly downstream of the RDN to control identity of photoreceptor
Rajam S Mani et al.
The Journal of biological chemistry, 294(2), 520-530 (2018-11-18)
Polynucleotide kinase/phosphatase (PNKP) and X-ray repair cross-complementing 1 (XRCC1) are key proteins in the single-strand DNA break repair pathway. Phosphorylated XRCC1 stimulates PNKP by binding to its forkhead-associated (FHA) domain, whereas nonphosphorylated XRCC1 stimulates PNKP by interacting with the PNKP
Hansruedi Mathys et al.
Nature, 570(7761), 332-337 (2019-05-03)
Alzheimer's disease is a pervasive neurodegenerative disorder, the molecular complexity of which remains poorly understood. Here, we analysed 80,660 single-nucleus transcriptomes from the prefrontal cortex of 48 individuals with varying degrees of Alzheimer's disease pathology. Across six major brain cell
Michael R Green et al.
Cold Spring Harbor protocols, 2017(5) (2017-05-04)
Measuring the concentration of DNA using fluorometry is more sensitive than spectrophotometry, allowing the detection of nanogram quantities of DNA. In this assay, DNA preparations of known and unknown concentrations are incubated with the fluorochrome Hoechst 33258. Absorption values for
Ismail Abdou et al.
Nucleic acids research, 43(2), 875-892 (2014-12-30)
In the current model of DNA SSBR, PARP1 is regarded as the sensor of single-strand breaks (SSBs). However, biochemical studies have implicated LIG3 as another possible SSB sensor. Using a laser micro-irradiation protocol that predominantly generates SSBs, we were able

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