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Merck
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文件

93337

Sigma-Aldrich

Trizma®乙酸盐

BioUltra, ≥99.0% (NT)

别名:

TRIS 乙酸盐, 三(羟甲基)氨基甲烷 乙酸盐

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About This Item

线性分子式:
NH2C(CH2OH)3 · CH3COOH
CAS号:
6850-28-8
分子量:
181.19
Beilstein:
3702918
EC號碼:
229-939-6
MDL號碼:
MFCD00038951
分類程式碼代碼:
12161700
PubChem物質ID:
57653535
NACRES:
NA.25

產品線

BioUltra

品質等級

300

化驗

≥99.0% (NT)

形狀

crystalline powder

雜質

insoluble matter, passes filter test

燃燒殘留物

≤0.2%

損耗

≤0.2% loss on drying, 20 °C (HV)

pH值

6.0-7.0 (25 °C, 0.5 M in H2O)

溶解度

H2O: 0.5 M at 20 °C, clear, colorless

負離子痕跡

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg

正離子痕跡

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

&lambda ；

0.5 M in H2O

紫外吸收

λ: 260 nm Amax: 0.07
λ: 280 nm Amax: 0.05

SMILES 字串

CC(O)=O.NC(CO)(CO)CO

InChI

1S/C4H11NO3.C2H4O2/c5-4(1-6,2-7)3-8;1-2(3)4/h6-8H,1-3,5H2;1H3,(H,3,4)

InChI 密鑰

PIEPQKCYPFFYMG-UHFFFAOYSA-N

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相关类别

應用

Trizma被用于配制pH范围在7.5和8.5之间的缓冲液。 Tris缓冲液被广泛用于细胞和分子生物学，如蛋白质和核酸提取和纯化等过程。 Trizma缓冲液也可用于柱层析和凝胶电泳。Trizma acetate用于制备Tris乙酸缓冲液，该缓冲液用作各种测定的稀释剂和电泳运行缓冲液。

其他說明

推荐的缓冲液，用于萤火虫荧光素酶ATP分析的最高灵敏度；谷氨酸结合试验

法律資訊

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

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Chi-Lin Li et al.
The Analyst, 137(22), 5222-5228 (2012-10-04)
Oligonucleotide (T30695) modified gold nanoparticles (T30695-Au NPs) have been prepared and employed for quantification of lead ions (Pb(2+)) in blood. The detection of Pb(2+) ions is through the formation of Au-Pb alloys and oligonucleotide-Pb(2+) complexes that catalyze the H(2)O(2)-mediated oxidation
Monica Cubillos-Rojas et al.
Electrophoresis, 31(8), 1318-1321 (2010-03-24)
To separate and analyze giant and small proteins in the same electrophoresis gel, we have used a 3-15% polyacrylamide gradient gel containing 2.6% of the crosslinker bisacrylamide and 0.2 M of Tris-acetate buffer (pH 7.0). Samples were prepared in a
Choice of buffer anion for the assay of adenosine 5'-triphosphate using firefly luciferase.
W W Nichols et al.
Analytical biochemistry, 114(2), 396-397 (1981-07-01)
M Ito et al.
Life sciences, 38(12), 1089-1096 (1986-03-24)
[3H]L-glutamic acid binding to microfuge tubes and glass was investigated in four buffers. Background binding to these materials was negligible, but was increased by centrifugation or suction in Tris-HCl and Tris-citrate buffer. This binding was much less or eliminated when
Monica Cubillos-Rojas et al.
Methods in molecular biology (Clifton, N.J.), 869, 205-213 (2012-05-16)
Polyacrylamide gel electrophoresis (PAGE) is one of the most powerful tools used for protein analysis. We describe the use of Tris-acetate buffer and 3-15% polyacrylamide gradient gels to simultaneously separate proteins in the mass range of 10-500 kDa. We show
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