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生物源
human
重組細胞
expressed in E. coli
描述
0.1 mg recombinant human E-Cadherin in 20 mM Tris-HCl buffer, containing NaCl, KCl, EDTA, L-arginine, DTT and glycerol.
無菌
Filtered sterilized solution
化驗
≥90% (SDS-PAGE)
形狀
liquid
包裝
pkg of 100 μg
濃度
0.5 mg protein/mL
技術
cell culture | mammalian: suitable
登錄號
NP_004351.1
運輸包裝
wet ice
儲存溫度
−20°C
基因資訊
human ... CDH1(999)
應用
上皮钙黏蛋白已作为包被基质蛋白,用于 1)维持长期 ES 或 iPS 细胞培养,然后与适当的 ES 细胞培养基相结合,2)作为包被基质材料,用于极低非特异相互作用的蛋白源 iPS 方案的11R标签重组 TF 细胞内传递,以及 3)作为天然抗原用于抗体生产。
以这些建议作为指导,确定您的培养系统的最佳包被条件。
1. 解冻上皮钙黏蛋白,用无血清培养基或 PBS 稀释至所需浓度。最终溶液应充分稀释,使加入的量均匀覆盖表面。
注:6 孔板中每孔使用 1 mL PBS。
2. 加入 5-10μg 蛋白至各孔,并在2~10°C 下培养一夜。
3. 培养后,抽吸剩余材料。
4. 孔板即时可用。可将其以潮湿状态储存在 2-8°C下或晾干(如果需要保持无菌)。
以这些建议作为指导,确定您的培养系统的最佳包被条件。
1. 解冻上皮钙黏蛋白,用无血清培养基或 PBS 稀释至所需浓度。最终溶液应充分稀释,使加入的量均匀覆盖表面。
注:6 孔板中每孔使用 1 mL PBS。
2. 加入 5-10μg 蛋白至各孔,并在2~10°C 下培养一夜。
3. 培养后,抽吸剩余材料。
4. 孔板即时可用。可将其以潮湿状态储存在 2-8°C下或晾干(如果需要保持无菌)。
序列
MDWVIPPISCPENEKGPFPKNLVQIKSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSNGNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVNTYNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRESFPTYTLVVQAADLQGEGLSTTATAVITVTDTNDNPPIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWRDTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPNTSPFTAELTHGASANWTIQYNDPTQESIILKPKMALEVGDYKINLKLMDNQNKDQVTTLEVSVCDCEGAAGVCRKAQPVEAGLQIP
準備報告
用密码子优化法构建重组人上皮钙黏蛋白基因(155-710 aa 断片),并以非融合蛋白形式在 大肠杆菌 中表达为包涵体。使用独特的“温度漂移包涵体复性”技术对最终产物进行复性和色谱纯化。
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 2
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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