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Key Documents

5085

Sigma-Aldrich

E-钙粘素 人

recombinant, expressed in E. coli, 0.5 mg protein/mL

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About This Item

分類程式碼代碼:
12352202
NACRES:
NA.75

生物源

human

重組細胞

expressed in E. coli

描述

0.1 mg recombinant human E-Cadherin in 20 mM Tris-HCl buffer, containing NaCl, KCl, EDTA, L-arginine, DTT and glycerol.

無菌

Filtered sterilized solution

化驗

≥90% (SDS-PAGE)

形狀

liquid

包裝

pkg of 100 μg

濃度

0.5 mg protein/mL

技術

cell culture | mammalian: suitable

登錄號

NP_004351.1

運輸包裝

wet ice

儲存溫度

−20°C

基因資訊

human ... CDH1(999)

應用

上皮钙黏蛋白已作为包被基质蛋白,用于 1)维持长期 ES 或 iPS 细胞培养,然后与适当的 ES 细胞培养基相结合,2)作为包被基质材料,用于极低非特异相互作用的蛋白源 iPS 方案的11R标签重组 TF 细胞内传递,以及 3)作为天然抗原用于抗体生产。

以这些建议作为指导,确定您的培养系统的最佳包被条件。
1. 解冻上皮钙黏蛋白,用无血清培养基或 PBS 稀释至所需浓度。最终溶液应充分稀释,使加入的量均匀覆盖表面。
注:6 孔板中每孔使用 1 mL PBS。
2. 加入 5-10μg 蛋白至各孔,并在2~10°C 下培养一夜。
3. 培养后,抽吸剩余材料。
4. 孔板即时可用。可将其以潮湿状态储存在 2-8°C下或晾干(如果需要保持无菌)。

序列

MDWVIPPISCPENEKGPFPKNLVQIKSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSNGNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVNTYNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRESFPTYTLVVQAADLQGEGLSTTATAVITVTDTNDNPPIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWRDTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPNTSPFTAELTHGASANWTIQYNDPTQESIILKPKMALEVGDYKINLKLMDNQNKDQVTTLEVSVCDCEGAAGVCRKAQPVEAGLQIP

準備報告

用密码子优化法构建重组人上皮钙黏蛋白基因(155-710 aa 断片),并以非融合蛋白形式在 大肠杆菌 中表达为包涵体。使用独特的“温度漂移包涵体复性”技术对最终产物进行复性和色谱纯化。

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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