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品質等級
描述
for research or for further manufacturing use
無菌
sterile-filtered
形狀
liquid
技術
cell culture | insect: suitable
成分
NaHCO3: 0.35 g/L
L-glutamine: no
glucose: 6 g/L
HEPES: no
運輸包裝
ambient
儲存溫度
2-8°C
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應用
EX-CELL 420 是一种完全培养基，针对 Sf9 和 Sf21 昆虫细胞系的无血清生长而开发和优化。细胞无需适应即可从无血清或加血清的培养基中直接转到 EX-CELL 420 中进行传代培养。EX-CELL 420 中的培养通常可达到高于 1×107 个/mL 的细胞密度，活性大于 95%。悬浮培养不添加培养基时可维持 10 天以上。Sf9 和 Sf21 细胞可在 EX-CELL 420 培养基中传代超过 20 次而不丧失活性。含血清培养基可提高蛋白质的表达和病毒的产量。
法律資訊
仅在美国、加拿大和欧洲地区出售。 有关其他国家/地区的可用性和限制，请咨询您当地的供应商。
EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
Hideki Yamaji et al.
Journal of bioscience and bioengineering, 95(2), 185-187 (2005-10-20)
When rat protein kinase C-delta (PKC-delta) was produced by Sf9 cells infected with a recombinant baculovirus in shake-flask culture using a serum-containing medium, the intracellular PKC-delta content decreased in the late period while the extracellular PKC-6 markedly increased. During the
Norikatsu Nishikawa et al.
Cytotechnology, 43(1-3), 3-10 (2008-11-13)
The production of beta-galactosidase by Sf9 cells infected with recombinant Autographa californica nucleopolyhedrovirus (AcNPV) was investigated in shake-flask culture using two serum-free basal media: Grace's medium and TNM-FH (Grace's medium supplemented with lactalbumin hydrolysate and yeast extract). At the time
Fabiana R X Batista et al.
Cytotechnology, 49(1), 1-9 (2008-11-13)
Animal cells can be cultured both in basal media supplemented with fetal bovine serum (FBS) and in serum-free media. In this work, the supplementation of Grace's medium with a set of nutrients to reduce FBS requirements in Spodoptera frugiperda (Sf9)
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