106-05F

Human Dermal Fibroblasts: HDF, fetal

• 分類程式碼代碼: 41106514
• NACRES: NA.81

属性

• 生物源: human neonatal foreskin or adult skin (normal)
• 品質等級: 100
• 包裝: pkg of 500,000 cells
• 製造商／商標名: Cell Applications, Inc
• 增長模式: Adherent
• 染色體組型: 2n = 46
• 形態學: Fibroblast
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

一般說明

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Dermal Fibroblasts are responsible for producing the extracellular matrix forming the connective tissue of the skin, and play a crucial role during wound healing. HDF provide an excellent model system to study many aspects of cell physiology, and have been utilized in dozens of research publications, particularly those related to skin biology and reprogramming/induced pluripotency studies. HDF were used to create, along with human synovyocytes, induced pluripotent stem cells (iPSC) by using the now classic “Yamanaka cocktail” of Oct3/4, Sox2, Klf4, and c-Myc, the discovery for which Dr. Shinya Yamanaka was awarded the Nobel Prize in 2012 (Takanashi, 2007; 2009), and to characterize iPSC general properties, miRNA expression profiles and differentiation potential (Hayashi, 2010; Ohta, 2011; Koyanagi-Aoi, 2013; Noguchi, 20130; Razak, 2013; Sakurai, 2013; Tanaka, 2013; Yanagida, 2013)

細胞系來源

Skin

應用

injury recovery, connective tissue research, laminin and fibronectin production, extracellular matrix, tissue organization, wound healing, fibroblasts growth factor response

成分

Fibroblast Basal Medium containing 10% FBS & 10% DMSO

準備報告

• Primary culture, >500,000 cells in Fibroblast Basal Medium containing 10% FBS & 10% DMSO
• Can be cultured at least 16 doublings

例行更新培養

Please refer to the HDF Culture Protocol.

免責聲明

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable