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07985

Sigma-Aldrich

6-氨基荧光素

BioReagent, suitable for fluorescence, ~95% (HPLC)

别名:

荧光素胺异构体II

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About This Item

经验公式（希尔记法）:
C20H13NO5
CAS号:
51649-83-3
分子量:
347.32
Beilstein:
51708
EC號碼:
257-334-7
MDL號碼:
MFCD00005051
分類程式碼代碼:
12171500
PubChem物質ID:
329748535
NACRES:
NA.32

產品線

BioReagent

化驗

~95% (HPLC)

形狀

powder

雜質

≤5% 5-aminofluorescein

mp

285 °C (dec.) (lit.)

溶解度

DMSO: soluble
acetone: soluble
methanol: soluble

螢光

λex 490 nm; λem 520 nm in 0.1 M Tris pH 9.0

適合性

suitable for fluorescence

SMILES 字串

Nc1ccc2C(=O)OC3(c4ccc(O)cc4Oc5cc(O)ccc35)c2c1

InChI

1S/C20H13NO5/c21-10-1-4-13-16(7-10)20(26-19(13)24)14-5-2-11(22)8-17(14)25-18-9-12(23)3-6-15(18)20/h1-9,22-23H,21H2

InChI 密鑰

YOAWSYSKQHLFPM-UHFFFAOYSA-N

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應用

6-氨基荧光素已被证明是基于富勒烯的脂质体纳米结构（称为'buckysomes'）的有效荧光标记试剂。溶解在0.1N NaOH中的6-氨基荧光素可通过490nm处的最大吸光度进行定量。溶解在6N HCl中的脱羧化的6-氨基荧光素（通过在110℃恒沸24小时制备）可通过454nm处的最大吸光度进行定量。醛基是通过对蛋白质的氧化损伤形成的，其可以通过与脱羧的6-氨基荧光素结合来检测。

其他說明

注意：不要与荧光胺混淆。

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Gloves

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I Climent et al.
Analytical biochemistry, 182(2), 226-232 (1989-11-01)
Oxidative modification of proteins is implicated in a number of physiologic and pathologic processes. Metal-catalyzed oxidative modification usually causes inactivation of enzymes and the appearance of carbonyl groups in amino acid side chains of the protein. We describe use of
K C Ingham et al.
Biochimica et biophysica acta, 670(2), 181-189 (1981-09-29)
A method for covalent attachment of a fluorescent molecule to the carbohydrate moieties of glycoproteins is described. The glycoproteins were oxidized with periodate under mild conditions selective for sialic acid (Van Lenten, L. and Ashwell, G. (1971) J. Biol. Chem.
Miju Kim et al.
ACS nano, 9(8), 8269-8278 (2015-07-15)
Polyelectrolyte microcapsules represent versatile stimuli-responsive structures that enable the encapsulation, protection, and release of active agents. Their conventional preparation methods, however, tend to be time-consuming, yield low encapsulation efficiency, and seldom allow for the dual incorporation of hydrophilic and hydrophobic
Thapakorn Tree-Udom et al.
ACS applied materials & interfaces, 7(43), 23993-24000 (2015-10-16)
Although computer simulation and cell culture experiments have shown that elongated spherical particles can be taken up into cells more efficiently than spherical particles, experimental investigation on effects of these different shapes over the particle-membrane association has never been reported.
Thomas Tigges et al.
Small (Weinheim an der Bergstrasse, Germany), 11(35), 4540-4548 (2015-06-06)
Patchy particles are next generation colloidal building blocks for self-assembly and find further use as (bio) sensors. Progress in this direction crucially depends on developing straightforward preparation pathways able to provide patchy particles with highest uniformity and integrating precise, orthogonal

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