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Merck

07376

Sigma-Aldrich

Atto 647 NHS酯

BioReagent, suitable for fluorescence, ≥90% (HPLC)

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About This Item

MDL號碼:
分類程式碼代碼:
12352108
NACRES:
NA.25

產品線

BioReagent

品質等級

化驗

≥90% (HPLC)
≥90% (degree of coupling)

製造商/商標名

ATTO-TEC GmbH

&lambda ;

in ethanol (with 0.1% trifluoroacetic acid)

紫外吸收

λ: 646-652 nm Amax

適合性

suitable for fluorescence

儲存溫度

−20°C

相关类别

應用

Atto 647 NHS 酯是一种使用 Atto 647 荧光染料对分子进行共价标记的试剂。Atto 647 属于红色光谱区域的新一代荧光标记物。该染料专用于生命科学领域,如标记 DNA、RNA 或蛋白质。该标记物的特征是吸附力强、荧光量子产率高、光稳定性高、水溶性好以及三重态形成非常少。Atto 647 是一种两性离子染料,其净电荷为零。Atto 647 具有 pH 敏感性。尽管在 pH 值高达 8.5 时几乎能够保持稳定,但在更高 pH环 境下会慢慢降解。
Atto 荧光标记专为高灵敏度应用设计,包括单分子检测。Atto 标记物具有刚性结构,不显示任何 顺式-反式异构化。因此,这些标记物表现出超常的强度,并且在共轭时具有极小的光谱移位。

法律資訊

本产品仅供研究使用。如果打算商业化,请联系知识产权持有者(德国ATTO-TEC GmbH公司)申请许可。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Breaking the Diffraction Barrier in Fluorescence Microscopy by Optical Shelving.
Bretschneider, S.; Eggeling, Chr.; Hell, S. W.
Physical Review Letters, 98(21) (2007)
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Biophysical journal, 93(9), 3120-3131 (2007-07-17)
We have developed a new method, using a nanopipette, for controlled voltage-driven delivery of individual fluorescently labeled probe molecules to the plasma membrane which we used for single-molecule fluorescence tracking (SMT). The advantages of the method are 1), application of
Jean-Manuel Segura et al.
The Journal of biological chemistry, 283(35), 24254-24263 (2008-06-27)
CD8(+) cytotoxic T lymphocytes (CTL) can recognize and kill target cells expressing only a few cognate major histocompatibility complex (MHC) I-peptide complexes. This high sensitivity requires efficient scanning of a vast number of highly diverse MHC I-peptide complexes by the
Daniel Puntener et al.
Journal of virology, 85(1), 481-496 (2010-11-05)
Human adenoviruses (Ads) replicate and assemble particles in the nucleus. They organize a linear double-strand DNA genome into a condensed core with about 180 nucleosomes, by the viral proteins VII (pVII), pX, and pV attaching the DNA to the capsid.
Glutamate-Induced AMPA Receptor Desensitization Increases Their Mobility and Modulates Short-Term Plasticity through Unbinding from Stargazin.
Constals, A., et al.
Neuron, 85(4), 707-803 (2015)

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