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一般說明
Tris buffer, pH 7.0 is a general buffer that is isotonic and non-toxic in nature due to the presence of tris base. It is suitable for most biochemical and molecular biology applications. Tris has a buffering capacity range of pH 7.0 - 9.0, with a pKa of 8.1 at 25°C.
應用
Tris buffer is used in:
- Western blot
- Protein gel electrophoresis
- cell culture (sterile filter buffer)
- As a buffer component
特點和優勢
- Fast, ready-to-use buffer
- No need to measure and adjust the pH
- Saves time and minimizes effort in the lab
- Biological tests: free of DNase, RNase, Protease, and Nickase
- Chemical tests: Iron ≤10 ppm, lead ≤5 ppm
包裝
Foil pouches
重構
Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 0.1M Tris solution, pH 7.0 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free. This product is suitable for use in cell culture and electrophoresis applications.
儲存和穩定性
Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.
其他說明
For additional information on our range of Biochemicals, please complete this form.
法律資訊
pHast Pack is a trademark of Sigma-Aldrich Co. LLC
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
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