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Merck

B0126

Sigma-Aldrich

溴酚蓝

Powder

别名:

3′,3′′,5′,5′′-四溴酚磺酰酞, 溴酚蓝磺酸内酯, 溴酚蓝(BPB)

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About This Item

经验公式(希尔记法):
C19H10Br4O5S
CAS号:
分子量:
669.96
Beilstein:
61698
EC號碼:
MDL號碼:
分類程式碼代碼:
12171500
PubChem物質ID:
NACRES:
NA.47

product name

溴酚蓝, titration: suitable

agency

suitable for SM 2310
suitable for SM 2320

品質等級

形狀

powder

技術

titration: suitable

顏色

faint beige to very dark beige
faint orange to very dark orange

visual transition interval

3.0-4.6, yellow-green to blue

mp

273 °C

溶解度

methanol: 10 mg/mL

應用

diagnostic assay manufacturing
hematology
histology

儲存溫度

room temp

SMILES 字串

Oc1c(Br)cc(cc1Br)C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4

InChI

1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H

InChI 密鑰

UDSAIICHUKSCKT-UHFFFAOYSA-N

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一般說明

溴酚蓝在酸性水溶液中以磺化羟基醌的形式存在,又称四溴酚磺酞。溴酚蓝是一种pH指示剂,并且是一种强蓝色染料。它具有轻微的负电荷并且可沿与DNA相同的方向迁移,允许用户对分子在凝胶中移动的进程进行监测。不同的凝胶组成具有不同的迁移速率。

應用

在 DNA、RNA(琼脂糖)和蛋白质(聚丙烯酰胺)凝胶电泳中,溴酚蓝用作示踪染料。它已用于为蛋白质印迹分析制备蛋白质样品。溴酚蓝在琼脂糖凝胶中以与300-500 bp DNA大致相同的速率迁移,在蛋白聚丙烯酰胺凝胶中位于缓冲前沿。此外,它还是为人熟知的重要血脑屏障检测染色剂。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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