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Merck

11759779001

Roche

BM Chemiluminescence ELISA Substrate (AP)

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About This Item

分類程式碼代碼:
12352204

用途

sufficient for 1,000 tests (ELISA)
sufficient for 600 tests (SEAP)

包裝

pkg of 150 mL

製造商/商標名

Roche

運輸包裝

wet ice

儲存溫度

2-8°C

特異性

The AP assay system is designed for measuring alkaline phosphatase of various sources (e.g. intestine, placenta, milk, bacteria).

特點和優勢

  • Sensitive: Approximately 10fg intestinal alkaline phosphatase can be detected
  • High dynamic measuring range: Linear range of more than five orders of magnitude
  • Constant light emission: The assay produces a long-lasting light emission instead of a short peak kinetics

成分

1. Alkaline Phosphatase Substrate, CSPD
2. Enhancer, Emerald II
3. Assay Buffer

原則

CSPD is dephosphorylated by alkaline phosphatase (AP). The resulting instable dioxetane anion decomposes and emits light at a maximum wavelength of 477nm. Chemiluminescence-enhancing reagents improve the quantum yield of the excited state by more than 500 fold.

準備報告

Working solution: Preparation of working solution

The substrate reagent is composed of AP-substrate (bottle 1), Enhancer (bottle 2) and Assay Buffer (bottle 3). Prepare substrate reagent freshly before use. The ratio of the components depends on the application. The substrate reagent is stable at least for one day at 2 to 8 °C.

其他說明

仅用于生命科学研究。不可用于诊断。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

does not flash

閃點(°C)

does not flash


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JunBae Jee et al.
Journal of clinical microbiology, 42(12), 5664-5672 (2004-12-08)
We investigated the acquisition and prevalence of Chlamydophila sp. infection in calves. Specimens were collected at weekly intervals from birth to week 12 postpartum from 40 female Holstein calf-dam pairs in a dairy herd. Real-time PCR detected, quantified, and differentiated
Martijn J Wilmer et al.
Cell and tissue research, 339(2), 449-457 (2009-11-11)
Reabsorption of filtered solutes from the glomerular filtrate and excretion of waste products and xenobiotics are the main functions of the renal proximal tubular (PT) epithelium. A human PT cell line expressing a range of functional transporters would help to

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