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生物源
human blood
品質等級
形狀
solution
包裝
pkg of 100 μg
製造商／商標名
Roche
濃度
0.2 mg/mL
顏色
colorless
溶解度
water: miscible
儲存溫度
2-8°C
一般說明
DNA is composed of units called nucleotides arranged into two long polynucleotide chains, resulting in a double helical structure. Nucleotides contain a phosphate group, a sugar group and a nitrogen base. There are four types of nitrogen bases namely adenine (A), thymine (T), guanine (G) and cytosine (C). The arrangement of these bases establishes the genetic codon. The difference in the nucleotide sequences is the reason why organisms differ from one another. In eukaryotic cell, the DNA is localized to the nucleus.
High molecular weight (>50kb) genomic DNA isolated from human blood (buffy coat) by the method of Sambrook et al..
應用
Human Genomic DNA is suitable for:
- Southern hybridization analysis
- genomic library construction
- the amplification of large DNA targets by the Expand System
- to assess the quality or integrity of DNA sample using qPCR or real time (RT) PCR and as a control during DNA sequencing
生化／生理作用
DNA is an essential component of the mechanism for heredity. The nucleotide sequences carry information regarding different biological processes. The genetic codons encode proteins essential for biological function. This genetic information is transmitted to the next generation during cell division. The amplification of very long fragments of genomic DNA requires template DNA of very high quality. In some cases, amplification failure may be due to poor template quality. This particular quality of genomic DNA is prepared to ensure reliable amplification of long DNA fragments.
品質
Molecular weight: The preparation is electrophoretically separated on a 0.5% agarose gel and the gel is stained with ethidium bromide. The molecular weight of the purified genomic DNA is greater than 50kb.
Function test: The preparation is used as template in a PCR with the Expand PCR System and appropriate primers from the human tPA Control Primer Set. Amplification products up to 27kb long are obtained.
Absence of contaminating organisms: The serum used for this preparation was tested for HBs antigen and the presence of antibodies to HIV-1, HIV-2, HCV. All tests were negative.
Function test: The preparation is used as template in a PCR with the Expand PCR System and appropriate primers from the human tPA Control Primer Set. Amplification products up to 27kb long are obtained.
Absence of contaminating organisms: The serum used for this preparation was tested for HBs antigen and the presence of antibodies to HIV-1, HIV-2, HCV. All tests were negative.
外觀
Solution in 10 mM Tris HCl, 1 mM EDTA, pH 8.0
其他說明
仅用于生命科学研究。不可用于诊断。
免責聲明
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
nwg
閃點（°F）
No data available
閃點（°C）
No data available
L M Rice et al.
Journal of applied microbiology, 115(3), 818-827 (2013-06-19)
The goal of this study was to develop a molecular diagnostic multiplex assay for the quantitative detection of microbial pathogens commonly responsible for ventilator-associated pneumonia (VAP) and their antibiotic resistance using linear-after-the-exponential polymerase chain reaction (LATE-PCR). This multiplex assay was
Alberts B, et al.
Molecular Cell Biology. 4th edition. (2002)
miR-29 acts as a decoy in sarcomas to protect the tumor suppressor A20 mRNA from degradation by HuR.
M Y Balkhi et al.
Science signaling, 6(286), ra63-ra63 (2013-08-01)
In sarcoma, the activity of NF-κB (nuclear factor κB) reduces the abundance of the microRNA (miRNA) miR-29. The tumor suppressor A20 [also known as TNFAIP3 (tumor necrosis factor-α-induced protein 3)] inhibits an upstream activator of NF-κB and is often mutated
Shana L McDevitt et al.
Biopreservation and biobanking, 12(6), 402-408 (2014-12-17)
Stable dry-state storage of DNA is desirable to minimize required storage space and to reduce electrical and shipping costs. DNA purified from various commercially available dry-state stabilization matrices has been used successfully in downstream molecular applications (e.g., quantitative polymerase chain
Ioannis G Fatouros et al.
Clinical chemistry, 52(9), 1820-1824 (2006-07-15)
Circulating free plasma DNA is implicated in conditions associated with tissue injury, including exercise-induced inflammation, and thus is a potential marker for athletic overtraining. We measured free plasma DNA along with C-reactive protein (CRP), creatine kinase (CK), and uric acid
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