SCR023

Endothelial Cell Characterization Kit

Endothelial cell characterization kits enable researchers to characterize cultured endothelial cells in both a constitutive & activated state using basic ICC techniques.

• 别名: Characterization Kit, Endothelial Cell Kit, Kit for Endothelial Characterization
• 分類程式碼代碼: 12352207
• eCl@ss: 32161000
• NACRES: NA.84

属性

• 品質等級: 100
• 物種活性: human
• 製造商／商標名: Chemicon^®
• 技術: cell culture | stem cell: suitable; immunocytochemistry: suitable
• 輸入: sample type: mouse embryonic stem cell(s); sample type neural stem cell(s); sample type hematopoietic stem cell(s); sample type epithelial cells; sample type pancreatic stem cell(s); sample type: human embryonic stem cell(s); sample type induced pluripotent stem cell(s); sample type mesenchymal stem cell(s); sample type cardiac stem cell(s)
• 運輸包裝: wet ice

说明

一般說明

Endothelial Cell Characterization Kit

Antibodies for the immunocytochemical characterization of endothelial cells.

Endothelial cells form the inner lining of blood vessels and serve as the cellular interface between the circulating blood and the vessel wall. Endothelial cells are involved in many aspects of vascular biology, including inflammation, angiogenesis, atherosclerosis, blood clotting, vasoconstriction and vasodilation. Chemicon′s endothelial cell characterization kit enables researchers to characterize cultured endothelial cells in both a constitutive and activated state using basic immunocytochemistry techniques.

Endothelial cells express the following markers constitutively. For optimal results, the following antibody dilutions are recommended for immunocytochemistry:

* Mouse anti-Endothelial Cell (CD146): 1/500 dilution based on 1 mg/mL, final 2 ng/mL

* Rabbit anti-VWF: 1/5000 dilution based on 1 mg/mL, final 0.2 ng/mL

* Mouse anti-CD31: 1/50 dilution based on 0.1 mg/mL, final 20 ng/mL

The following antibodies have been shown to stain cultured HUVEC after 4-6hr of TNF-alpha (100ng/ml) induction at the following dilutions:

* Mouse anti-ICAM-1: 1/500 dilution based on 1 mg/mL, final 2 ng/mL

* Mouse anti-VCAM-1: 1/500 dilution based on 1 mg/mL, final 2 ng/Ml

應用

Research Category
Stem Cell Research

成分

50 μg of Endothelial Cell (CD146) monoclonal (Part No. 2003789)

50 μg of CD31 monoclonal (Part No. 2003788)

10 μg of von Willibrand Factor (vWF) polyclonal (Part No. 2003787)

50 μg of VCAM-1 monoclonal (Part No.MAB2511-50UG)

50 μg of ICAM-1 monoclonal (Part No. 2003785)

100 μg of Mouse control IgG (Part No. PP54-100UG)

100 μg of Rabbit control IgG (Part No. PP64-100UG)

儲存和穩定性

Antibodies to ICAM-1, VCAM-1, von Willibrand Factor (vWF), and Endothelial Cells (CD146) are to be stored at 2 - 8°C (SCR023 - Part 1). Antibodies to CD31, Mouse IgG, and Rabbit IgG are to be stored at -20°C (SCR023 - Part 2).

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 10 - Combustible liquids