推荐产品
product name
N27 Rat Dopaminergic Neural Cell Line, The N27 rat dopaminergic neural cell line has been used as a dopaminergic neuron model for in vitro and in vivo studies.
生物源
rat
品質等級
技術
cell culture | stem cell: suitable
運輸包裝
liquid nitrogen
一般說明
Parkinson’s disease is a neurodegenerative disorder of the central nervous system that affects more than 6 million people worldwide. The motor symptoms of Parkinson′s disease result from the death of dopamine generating cells in the substantia nigra, a region of the midbrain. Numerous efforts have been made to slow the loss or replace these dopamine producing neurons in vivo.
The N27 rat dopaminergic neuron cell line was harvested from E12 rat mesencephalic tissue and was transfected with SV40 to immortalize the cell line. The N27 cell line, when injected into the striata of 6-hydroxydopamine-lesioned rats (an animal model of PD) caused a time-dependent improvement in neurological deficits. This immortalized cell line has been carefully characterized in studies of dopamine biosynthesis, neurotoxicity and used as a dopaminergic neuron model for in vitro and in vivo studies.
細胞系描述
Neural Stem Cells
Neural Lineage Cells
Neural Lineage Cells
應用
Research Category
Stem Cell Research
Neuroscience
Stem Cell Research
Neuroscience
Research Sub Category
Neural Stem Cells
Neurochemistry & Neurotrophins
Neurodegenerative Diseases
Neuroregenerative Medicine
Neural Stem Cells
Neurochemistry & Neurotrophins
Neurodegenerative Diseases
Neuroregenerative Medicine
This product is intended for sale and sold solely for internal non-commercial research use per the terms of the “Restricted Use Agreement” as detailed in the product documentation. For information regarding any other uses, please contact licensing@emdmillipore.com.
品質
• Each vial contains ≥ 1X106 viable cells.
• Cells are negative for mycoplasma contamination.
• Cells are negative for mycoplasma contamination.
儲存和穩定性
N27 cells should be stored in liquid nitrogen. The cells can be passage for at least 10 passages without significantly affecting the cell marker expression and functionality.
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Establishment and characterization of immortalized clonal cell lines from fetal rat mesencephalic tissue.
Prasad, K N, et al.
In Vitro Cellular & Developmental Biology. Animal, 30A, 596-603 (1994)
Improvement of neurological deficits in 6-hydroxydopamine-lesioned rats after transplantation with allogeneic simian virus 40 large tumor antigen gene-induced immortalized dopamine cells.
Clarkson, E D, et al.
Proceedings of the National Academy of Sciences of the USA, 95, 1265-1270 (1998)
M Terashvili et al.
Neuroscience, 223, 68-76 (2012-08-07)
Astrocytes perform several functions that are essential for normal neuronal activity. They play a critical role in neuronal survival during ischemia and other degenerative injuries and also modulate neuronal recovery by influencing neurite outgrowth. In this study, we investigated the
Fatema Bhinderwala et al.
Methods in molecular biology (Clifton, N.J.), 1996, 217-257 (2019-05-28)
Metabolomics has been successfully applied to study neurological and neurodegenerative disorders including Parkinson's disease for (1) the identification of potential biomarkers of onset and disease progression; (2) the identification of novel mechanisms of disease progression; and (3) the assessment of
C-terminal α-synuclein truncations are linked to cysteine cathepsin activity in Parkinson's disease.
Ryan P McGlinchey et al.
The Journal of biological chemistry, 294(25), 9973-9984 (2019-05-17)
A pathological feature of Parkinson's disease (PD) is Lewy bodies (LBs) composed of α-synuclein (α-syn) amyloid fibrils. α-Syn is a 140 amino acids-long protein, but truncated α-syn is enriched in LBs. The proteolytic processes that generate these truncations are not
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门