PK1010

PhosphoDetect Anti-Syk (pTyr^525/526) Rabbit pAb

liquid, Calbiochem^®

• 分類程式碼代碼: 12352203
• NACRES: NA.41

属性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 形狀: liquid
• 不包含: preservative
• 物種活性: human, mouse
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze

说明

一般說明

Protein A and immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~72 kDa Syk protein phosphorylated on Tyr^525/526.

Recognizes the ~72 kDa Syk protein phosphorylated at Tyr^525/526 in human Syk or Tyr^519/520 in mouse Syk in 293T cells expressing wild-type Syk and CD8.

This PhosphoDetect Anti-Syk (pTyr^525/526) Rabbit pAb is validated for use in ELISA, Immunoblotting for the detection of Syk (pTyr^525/526).

免疫原

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr^525/526 phosphorylation sites of human Syk

應用

ELISA (1:1000)
Immunoblotting (1:1000)

警告

Toxicity: Standard Handling (A)

外觀

In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.

重構

Do not aliquot.

分析報告

Positive Control
293T cells transfected with wild-type Syk and CD8

其他說明

Coopman, P.J., et al. 2000. Nature406, 742.
Turner, M., et al. 2000. Immunol. Today21, 148.
Zang, J., et al. 2000. J. Biol. Chem.275, 35442.
Chu, D.H., et. al. 1998. Immunol. Rev.165, 167.
Cheng, A.M. and Chan, A.C. 1997. Curr. Opin. Immunol.9, 528.
Kurosaki, T. 1997. Curr. Opin. Immunol.9, 309.

Tyr^525/526 represent the phosphorylation sites in human Syk, while Tyr^519/520 are the equivalent phosphorylation sites in mouse Syk. For immunoblotting the membrane should be incubated with antibody diluted in 5% BSA, 1X TBS, 0.1% Tween^®-20 detergent at 4°C overnight with gentle shaking. Antibody should be titrated for optimal results in individual systems.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2