生物源
rabbit
品質等級
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
liquid
不包含
preservative
物種活性
rat, mouse, human
製造商／商標名
Calbiochem®
儲存條件
OK to freeze
同型
IgG
運輸包裝
wet ice
儲存溫度
−20°C
目標翻譯後修改
phosphorylation (pSer15)
基因資訊
human ... TP53(7157)
一般說明
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~53 kDa p53 protein phosphorylated on Ser15.
Recognizes the ~53 kDa p53 protein phosphorylated at Ser15 in PC12 or HeLa cells treated with DNA damaging agents. Does not recognize unphosphorylated p53.
This PhosphoDetect Anti-p53 (pSer¹⁵) (Ab-3) Rabbit pAb is validated for use in Immunoblotting , Immunoprecipitation for the detection of p53 (pSer¹⁵) (Ab-3).
免疫原
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵ phosphorylation site of human p53, conjugated to KLH
應用
Immunoblotting (1:1000)
Immunoprecipitation (1:200)
警告
Toxicity: Standard Handling (A)
外觀
In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
重構
Following initial thaw, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
分析報告
Positive Control
PC-12, HCT116, or HeLa cells treated with DNA damaging agents
PC-12, HCT116, or HeLa cells treated with DNA damaging agents
其他說明
Does not recognize non-phosphorylated p53. Antibody should be titrated for optimal results in individual systems.
Tibbetts, R.S., et al. 1999. Genes Dev.13, 152.
Levine, A.J. 1997. Cell88, 323.
Milczarek, G.J., et al. 1997. Life Sci.60, 1.
Shieh, S.Y., et al. 1997. Cell91, 325.
Meek, D.W. 1994. Semin Cancer Biol.5, 203.
Levine, A.J. 1997. Cell88, 323.
Milczarek, G.J., et al. 1997. Life Sci.60, 1.
Shieh, S.Y., et al. 1997. Cell91, 325.
Meek, D.W. 1994. Semin Cancer Biol.5, 203.
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
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