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Key Documents

NA46

Sigma-Aldrich

Anti-p300 Mouse mAb (NM-11)

liquid, clone NM-11, Calbiochem®

别名:

Anti-p300 antibody

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

mouse

品質等級

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

NM-11, monoclonal

形狀

liquid

不包含

preservative

物種活性

human, rat

應無反應活性

mouse

製造商/商標名

Calbiochem®

儲存條件

OK to freeze
avoid repeated freeze/thaw cycles

同型

IgG1

運輸包裝

wet ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

human ... EP300(2033)

一般說明

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the p300 protein.
Recognizes the p300 protein in A431, HeLa, and ML-1 cells.
This Anti-p300 Mouse mAb (NM-11) is validated for use in Frozen Sections, Immunoblotting, Immunofluorescence, Immunoprecipitation, Paraffin Sections for the detection of p300.

免疫原

Epitope: within amino acids 2071-2091 near the CBP C-terminus
Human
recombinant, human p300 protein

應用

Frozen Sections (see application references)

Immunoblotting (1 µg/ml)

Immunofluorescence (1-5 µg/ml)

Immunoprecipitation (1 µg/sample)

Paraffin Sections (1-5 µg/ml, heat pre-treatment required)

包裝

Please refer to vial label for lot-specific concentration.

警告

Toxicity: Standard Handling (A)

外觀

In 0.05 M sodium phosphate buffer, 50% glycerol.

重構

Following initial thaw, aliquot and freeze (-20°C).

分析報告

Positive Control
A431, HeLa, or ML-1 cells

其他說明

Antibody should be titrated for optimal results in individual systems.
Dallas, P.B., et al. 1997. Hybridoma16, 273.
Shisler, J., et al. 1996. J. Virol.70, 68.
Wang, H.G., et al. 1995. J. Virol.69, 7917.
Banerjee, A.C.,et al. 1994. Oncogene9, 1733.
Eckner, R., et al. 1994. Genes Dev.8, 869.
Abraham, S.E., et al. 1993. Oncogene8, 1639.
Wang, H.G., et al. 1993. J. Virol.67, 476.
Howe, J.A., and Bayley, S.T., 1992. Virology186, 15.
Rikitake, Y., and Moran, E., 1992. Mol. Cell. Biol.11, 2826.
Yaciuk, P., and Moran, W., 1991. Mol. Cell. Biol.11, 5389.
Egan, C., et al. 1988. Mol. Cell. Biol.8, 3955.
Yee, S. and Branton, P., 1985. Virology147, 142.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3


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