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MHSTCMAG-70K

Millipore

MILLIPLEX®小鼠超敏T细胞组套 - 免疫学多重检测试剂盒

Simultaneous analyze low levels of cytokine and chemokine biomarker with the High Sensitivity Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.

别名:

Luminex® Mouse Immunology Panel, Millipore Mouse Immunology Panel, Mouse Immunology Protein Multiplex Assay

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About This Item

分類程式碼代碼:
12161503
eCl@ss:
32161000

品質等級

物種活性

mouse

製造商/商標名

Milliplex®

assay range

accuracy: 82-102%
sensitivity: 0.26-11.14 pg/mL
standard curve range: 0.10-400 pg/mL
(IL-4)

standard curve range: 0.49-2,000 pg/mL
(IFNγ)

standard curve range: 0.49-2,000 pg/mL
(IL-17A)

standard curve range: 0.49-2,000 pg/mL
(IL-7)

standard curve range: 0.49-2,000 pg/mL
(TNFα)

standard curve range: 0.73-3,000 pg/mL
(KC/GROα/CXCL1)

standard curve range: 0.98-4,000 pg/mL
(IL-2)

standard curve range: 0.98-4,000 pg/mL
(IL-5)

standard curve range: 0.98-4,000 pg/mL
(IL-6)

standard curve range: 1.34-5,500 pg/mL
(IL-10)

standard curve range: 2.93-12,000 pg/mL
(IL-12 (p70))

standard curve range: 3.17-13,000 pg/mL
(LIX)

standard curve range: 3.42-14.000 pg/mL
(IL-13)

standard curve range: 3.91-16,000 pg/mL
(IL-1α)

standard curve range: 3.91-16,000 pg/mL
(IL-1β)

standard curve range: 3.91-16,000 pg/mL
(MCP-1/CCL2)

standard curve range: 6.1-25,000 pg/mL
(GM-CSF)

standard curve range: 7.32-30,000 pg/mL
(MIP-2/CXCL2)

inter-assay cv: <15%
intra-assay cv: <10%

技術

multiplexing: suitable

檢測方法

fluorometric (Luminex xMAP)

運輸包裝

ambient

一般說明

细胞因子是在适应性和先天免疫应答中起关键作用的免疫调节多肽。通用术语“细胞因子”包括肌动蛋白(由肌肉细胞产生)、淋巴因子(由活化的Th细胞产生)、白介素(充当T细胞之间的介质)和趋化因子(负责T细胞迁移)。细胞因子是免疫系统的调节机制之一,作用于免疫应答的识别、激活或效应阶段,调节T细胞、B细胞和髓样细胞亚型的发育和功能活性。涉及细胞因子的研究对于更深入地了解免疫系统及其对大多数抗原的多方面反应,特别是那些组成炎症过程的反应,具有重要作用。

低水平的炎症涉及许多临床和亚临床疾病状态,例如自身免疫性疾病、心血管疾病、糖尿病、神经系统疾病和癌症。因此,测量细胞因子的皮克水平对于理解这些疾病的发病机理至关重要。

组套类型:细胞因子/趋化因子

特異性

交叉反应性
组套内无可检测的交叉反应性。
本组套中抗体与任何其他分析物之间的交叉反应性不可检出或可忽略不计。

應用

  • 分析物:GM-CSF、IFNγ、IL-1α、IL-1β、IL-2、IL-4、IL-5、IL-6、IL-7、IL-10、IL-12(p70)、IL-13、IL-17A、KC/CXCL1、LIX、MCP-1、MIP-2、TNF-α
  • 建议的样品类型:血清、血浆、组织培养物或其他培养基
  • 建议的样品稀释度:纯品或1:2稀释的血清或血浆
  • 测定运行时间:过夜
  • 研究类别:炎症&免疫学
  • 研究子类别:免疫反应代谢紊乱慢性炎症性疾病心血管疾病

特點和優勢

通过在此组套中选择可用的分析物来设计多重试剂盒。

儲存和穩定性

试剂盒组件的建议储存温度为2 - 8°C。

其他說明

灵敏度:单个分析物的灵敏度请参见方案。
请联系技术服务部了解稀释线性。

法律資訊

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

除非我们的目录或产品随附的其他公司文件中另有说明,否则我们的产品预期仅用于研究用途,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或对人类或动物的任何类型的消费或应用。

訊號詞

Danger

危險分類

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

標靶器官

Respiratory Tract

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3


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Increasing evidence indicates that inflammatory responses may influence brain neurochemical pathways, inducing depressive-like behaviors. Ultrasound stimulation (US) is a promising non-invasive treatment for neuropsychiatric diseases. We investigated whether US can suppress inflammation and improve depressive-like behaviors. Mice were intraperitoneally injected
Robert A Kazmierczak et al.
Oncotarget, 11(44), 3943-3958 (2020-11-21)
Conventional cancer chemotherapies are not fully efficacious and do not target tumors, leading to significant treatment-related morbidities. A number of genetically attenuated cancer-targeting bacteria are being developed to safely target tumors in vivo. Here we report the toxicological, tumor-targeting, and
Yingxin Sun et al.
Frontiers in cellular and infection microbiology, 11, 618265-618265 (2021-04-06)
Aging induced chronic systemic inflammatory response is an important risk factor for atherosclerosis (AS) development; however, the detailed mechanism is yet to be elucidated. To explore the underlying mechanism of how aging aggravates AS advancement. A young (five-week-old, YM) and
Hikaru Ito et al.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, 46(2), 442-454 (2020-09-18)
The lateral habenula (LHb) attracts a growing interest as a regulator of monoaminergic activity which were frequently reported to be defective in depression. Here we found that chronic social defeat stress (CSDS) increased production of pro-inflammatory cytokines in LHb associated
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PLoS pathogens, 16(12), e1009096-e1009096 (2020-12-15)
Bacille Calmette-Guerin (BCG), an attenuated whole cell vaccine based on Mycobacterium bovis, is the only licensed vaccine against Mycobacterium tuberculosis (Mtb), but its efficacy is suboptimal and it fails to protect against pulmonary tuberculosis. We previously reported that Mtb lacking

相关内容

MILLIPLEX®小鼠高灵敏度T细胞多因子检测试剂盒是对生物样本进行细胞因子谱分析的强大工具。了解该试剂盒的应用评价,包括细胞因子低水平表达研究,以及细胞因子分泌水平的多指标(多重、多因子)并行定量分析。

The MILLIPLEX® Mouse High Sensitivity T Cell Multiplex Panel is a powerful tool for cytokine profiling in biological samples. Uncover details on how this panel was evaluated for studying low-level cytokine expression and also quantifying multiple cytokine secretion levels simultaneously.

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