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生物源
mouse
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
9C2.1, monoclonal
物種活性
mouse, rat
物種活性（以同源性預測）
human (based on 100% sequence homology)
技術
immunohistochemistry: suitable
western blot: suitable
同型
IgG2bκ
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... TNNT2(7139)
一般說明
Troponin T, cardiac muscle (UniProt P45379; also known as Cardiac muscle troponin T, cTnT, TnTc) is encoded by the TNNT2 (also known as CMD1D, CMH2, RCM3) gene (Gene ID 7139) in human. Troponin is a complex composed of three regulatory proteins, troponin C, troponin I, and troponin T). Troponin is found in both skeletal muscle and cardiac muscle where it plays an integral role in muscle contraction. Troponin T (TnT) is the component that binds tropomyosin, while troponin I (TnI) binds actin in thin myofilaments to hold the actin-tropomyosin complex in place. Three tissue TnT types exist, the slow skeletal troponin T1 encoded by the TNNT1 gene, the cardiac troponin T2 encoded by the TNNT2 gene, and the fast skeletal troponin T3 encoded by the TNNT3 gene.
特異性
Expected to react with human troponin T spliced isoforms 1-8 and 10, as well as all four of mouse troponin T spliced isoforms (Embryonic, A1, A2, and A3B isoforms; UniProt P50752) and both of rat troponin T spliced isoforms (Embryonic and Adult isoforms; UniProt P50753)
免疫原
Epitope: Internal.
Recombinant human cardiac muscle troponin T internal fragment.
應用
Detect Troponin T using this Anti-Troponin T (Cardiac Muscle) Antibody, clone 9C2.1 validated for use in Western Blotting, Immunohistochemistry.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Troponin T (Cardiac Muscle) in rat cardiac muscle tissue.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
品質
Evaluated by Western Blotting in mouse heart tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Troponin T (Cardiac Muscle) in 10 µg of mouse heart tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Troponin T (Cardiac Muscle) in 10 µg of mouse heart tissue lysate.
標靶描述
~36 kDa observed. 35.8 kDa (isoform Embryonic), 35.0 kDa (isoform A1), 34.5 kDa (isoform A2), and 34.2 kDa (Isoform A3B) of mouse cardiac muscle troponin T spliced isoforms calculated.
外觀
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at 2-8°C from date of receipt.
其他說明
Concentration: Please refer to lot specific datasheet.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
Matthew L Klos et al.
Biomolecules, 12(10) (2022-10-28)
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