MABN681

Anti-TrkA Antibody, clone 6B2

ascites fluid, clone 6B2, from mouse

• 别名: High affinity nerve growth factor receptor, Neurotrophic tyrosine kinase receptor type 1, TRK1-transforming tyrosine kinase protein, Tropomyosin-related kinase A, Tyrosine kinase receptor, Tyrosine kinase receptor A, Trk-A, gp140trk, p140-TrkA
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: ascites fluid
• 抗體產品種類: primary antibodies
• 無性繁殖: 6B2, monoclonal
• 物種活性: human, rat
• 技術: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• 同型: IgG1
• NCBI登錄號: NP_002520
• UniProt登錄號: P04629
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... NTRK1(4914)

说明

一般說明

TrkA, apparent molecular weight 140 kDa, is a high affinity nerve growth factor (NGF). The Trk proto-oncogene family contains four members, TrkA, TrkB, TrkC, and TrkE, which are variably expressed throughout the central and peripheral nervous systems. TrkA binds to nerve growth factor (NGF) and autophosphorylates on tyrosine residues (Tyr490, Tyr674, Tyr675, Tyr751 and Tyr785) to activate multiple downstream effector proteins. Phosphorylation at Tyr490 is required for Shc association and subsequent activation of the Ras-MAP kinase-signaling cascade, which leads to activation of Elk-1-dependent gene transcription and neurite growth. Phosphorylations at Tyr674 and Tyr675 lie within the catalytic domain of TrkA tyrosine kinase and reflect Trk kinase activity. Additionally, phosphorylation at Tyr751 is required for PI3-kinase association and activation of the Akt signaling cascade.

免疫原

Purified recombinant extracellular fragment of TrkA expressed in HEK293 cells.

應用

Anti-TrkA Antibody, clone 6B2 is a highly specific mouse monoclonal antibody, that targets TrkA & has been tested in western blotting, IHC & ICC.

Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected TrkA in human brain and rat cerebellum tissues.

Immunocytochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected TrkA in PC-12 cells.

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

品質

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected TrkA in 10 µg of human brain tissue lysate.

標靶描述

~87 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 4 isoforms ranging between ~77 kDa and ~87 kDa

外觀

Unpurified

Mouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析報告

Control
Human brain tissue lysate

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: nwg
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable