MABN627

Anti-SDHB Antibody, clone 10C5.1

clone 10C5.1, from mouse

• 别名: Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial, SDHB, Iron-sulfur subunit of complex II, Ip
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702

属性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 10C5.1, monoclonal
• 物種活性: human, mouse
• 技術: immunohistochemistry: suitable (paraffin); western blot: suitable
• 同型: IgG2aκ
• NCBI登錄號: NP_002991
• UniProt登錄號: P21912
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... SDHB(6390)

说明

一般說明

Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (EC 1.3.5.1; UniProt P21912; also known as Ip, Iron-sulfur subunit of complex II) is encoded by the SDHB (also known as IP, SDH, CWS2, PGL4, SDH1, SDHIP) gene (Gene ID 6390) in human. Originally described by Otto Warburg and now commonly known as the Warburg effect refers to the phenomenon that many tumor cells use aerobic glycolysis instead of mitochondrial respiration for energy production to support cell growth and proliferation. Enzymes involved in the complex network of metabolic pathways are therefore being studied as potential cancer markers and targets for treatment. Succinate dehydrogenase (SDH), also known as mitochondrial respiratory Complex II, is a holoenzyme consisting of four essential subunits, SDHA (a flavoprotein), SDHB (iron-sulfur protein), and two membrane anchor units SDHC and SDHD. SDH assembly requires two factors, SDHAF1 and SDHAF2, while its function is influenced by the deacetylase activity of SIRT3. SDH is the only enzyme complex known to participate in both the TCA cycle and electron transport chain (ETC.). SDH catalyzes the oxidation of succinate in the TCA to form fumarate, a reaction that is coupled to the reduction of ubiquinone to ubiquinol in the ETC. SDH loss-of-function mutations have been linked to pheochromocytoma (PCC), paraganglioma (PGL), gastrointestinal stromal tumor (GIST), renal cell carcinoma, and ovarian cancer.

免疫原

Epitope: C-terminal half

GST-tagged recombinant protein corresponding to the C-terminal half of human SDHB.

應用

Anti-SDHB Antibody, clone 10C5.1 is an antibody against SDHB for use in Western Blotting, Immunohistochemistry (Paraffin).

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected SDHB in 10 µg of Jurkat cell lysate.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected SDHB in human heart, human small intestine, and mouse kidney tissue.

品質

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected SDHB in 10 µg of HEK293 cell lysate.

標靶描述

~30 kDa observed

外觀

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable