MABN543

Anti-Gs protein Antibody, alpha subunit, clone N192/12

clone N192/12, from mouse

• 别名: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short, Adenylate cyclase-stimulating G alpha protein
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: N192/12, monoclonal
• 物種活性: rat, human
• 物種活性（以同源性預測）: bovine (immunogen homology)
• 技術: western blot: suitable
• 同型: IgG1κ
• NCBI登錄號: NP_851364
• UniProt登錄號: P04896
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... GNAS(2778)

说明

一般說明

The heterotrimeric guanine nucleotide-binding regulatory proteins (G proteins) mediate signaling from a large number of G-protein coupled receptors (GPCR) to a variety of intracellular effectors. G-proteins are composed of a 36-52 kDa α-subunit, a 35-36 kDa β-subunit, and an 8-10 kDa γ-subunit. They are classified based on the nature of their α-subunit. Gs is ubiquitously expressed and couples receptors to adenylyl cyclase and promotes intracellular cAMP generation. Mutations in Gs-α can lead to constitutive activation of receptors leading to development of endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. In brain tissue Gs-α activates adenyl cyclase in direct response to stimulation of several neurotransmitter receptors and facilitates depolarization-induced neurotransmitter release for synaptic transmission. Also, it is shown that Gs-stimulated adenylyl cyclase activity is significantly reduced in the Alzheimer′s disease brain tissue.

特異性

Other homologies: Mouse (99% sequence homology).

免疫原

Recombinant protein corresponding to bovine GS protein, alpha subunit.

應用

Anti-Gs protein Antibody, alpha subunit, clone N192/12 is a highly specific mouse monoclonal antibody & that targets Gsα & has been tested in western blotting.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Western Blotting Analysis: 2.0 µg/mL from a representative lot detected GS protein, alpha subunit in 10 µg of SH-SY5Y cell lysate.

品質

Evaluated by Western Blotting in rat brain membrane tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected GS protein, alpha subunit in 10 µg of rat brain membrane tissue lysate.

標靶描述

~45 kDa observed

外觀

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
Rat brain membrane tissue lysate

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable