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生物源
mouse
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
14D5, monoclonal
物種活性
rat, opossum, bovine, mouse, human
技術
ELISA: suitable
immunofluorescence: suitable
western blot: suitable
同型
IgG2aκ
NCBI登錄號
UniProt登錄號
運輸包裝
ambient
目標翻譯後修改
phosphorylation (pSer552)
基因資訊
rat ... Slc9A3(24784)
一般說明
Sodium/hydrogen exchanger 3 (UniProt: P26433; also known as Na(+)/H(+) exchanger 3, NHE-3, Solute carrier family 9 member 3) is encoded by the Slc9a3 (Nhe3) gene (Gene ID: 24784) in rat. NHE-3 is a multi-pass membrane protein that is involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. It is a major proton extruding system driven by the inward sodium ion chemical gradient. NHE-3 is shown to be most abundant in colon and small intestine and is also present in stomach and in proximal tubes and outer medulla of kidney. In kidneys it mediates the majority of sodium bicarbonate reabsorption by the proximal tubules. NHE-3 can be phosphorylated and regulated by multiple kinases, including PKA, SGK1, and CK2 and its phosphorylation is critical in its regulation. Parathyroid hormone is shown to enhance PKA-mediated phosphorylation of Serine 552, however, this phosphorylation does not affect the activity of NHE-3. (Ref.: Kocinsky, HS., et al. (2007). Am J Physiol Renal Physiol 293 (1); F212-F218).
特異性
This mouse monoclonal antibody (clone 14D5) specifically detects Sodium/hydrogen exchanger 3 phosphorylated on Serine 552.
免疫原
KLH-conjugated linear peptide corresponding to 22 amino acids surrounding phospho Serine 552 from the C-terminal half of rat Sodium/hydrogen exchanger 3 (NHE3).
應用
Anti-phospho-NHE3 (Ser552), clone 14D5, Cat. No. MABN2415, is a highly specific mouse monoclonal antibody that targets Sodium/hydrogen exchanger 3 and has been tested for use in ELISA, Immunofluorescence, and Western Blotting.
Immunofluorescence Analysis: A representative lot detected phospho-NHE3 (Ser552) in Immunofluorescence applications (Kocinsky, H.S., et. al. (2005). Am J Physiol Renal Physiol. 289(2):F249-58).
Western Blotting Analysis: A representative lot detected phospho-NHE3 (Ser552) in Western Blotting applications (Kocinsky, H.S., et. al. (2005). Am J Physiol Renal Physiol. 289(2):F249-58; Kocinsky, H.S., et. al. (2007). Am J Physiol Renal Physiol. 293(1):F212-8).
ELISA Analysis: A representative lot detected phospho-NHE3 (Ser552) in ELISA applications (Kocinsky, H.S., et. al. (2005). Am J Physiol Renal Physiol. 289(2):F249-58).
Western Blotting Analysis: A representative lot detected phospho-NHE3 (Ser552) in Western Blotting applications (Kocinsky, H.S., et. al. (2005). Am J Physiol Renal Physiol. 289(2):F249-58; Kocinsky, H.S., et. al. (2007). Am J Physiol Renal Physiol. 293(1):F212-8).
ELISA Analysis: A representative lot detected phospho-NHE3 (Ser552) in ELISA applications (Kocinsky, H.S., et. al. (2005). Am J Physiol Renal Physiol. 289(2):F249-58).
Research Category
Neuroscience
Neuroscience
品質
Evaluated by Western Blotting in COS-7 cells either transfected with wild type NHE3, mutated NHE3 (S552A), or untransfected COS-7 (WT) cell lysates.
Western Blotting Analysis: 2 µg/mL of this antibody detected Sodium/hydrogen exchanger 3 in lysate from COS-7 cells transfected with wild- type NHE-3 (pSer552). It did not detect NHE-3 in lysate from untransfected COS-7 cells or those transfected with mutated NHE-3 (S552A).
Western Blotting Analysis: 2 µg/mL of this antibody detected Sodium/hydrogen exchanger 3 in lysate from COS-7 cells transfected with wild- type NHE-3 (pSer552). It did not detect NHE-3 in lysate from untransfected COS-7 cells or those transfected with mutated NHE-3 (S552A).
標靶描述
~85 kDa observed; 93.11 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
外觀
Protein G purified
Format: Purified
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at 2-8°C from date of receipt.
其他說明
Concentration: Please refer to lot specific datasheet.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
does not flash
閃點（°C）
does not flash
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