推荐产品
生物源
rat
品質等級
共軛
violetFluor® 450
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
RA3-6B2, monoclonal
物種活性
human, mouse
製造商/商標名
violetFluor®
濃度
0.2 mg/mL
技術
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同型
IgG2aκ
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... PTPRC(5788)
mouse ... Ptprc(19264)
一般說明
The RA3-6B2 antibody reacts with the human and mouse CD45 isoform known as CD45R, or B220, a protein tyrosine phosphatase of 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns, all critical for leukocyte function. In mouse, the CD45R/B220 isoform is predominantly found on B cells, at varying levels on all stages from pro-B cells to activated B cells, and may also be detected on certain T cell and NK cell subsets. It is of note that B220 is not similarly expressed on human B cells, where it appears to be differentiation-specific and therefore expressed on only some B cell subsets. Other forms of CD45 with restricted cellular expression include CD45RA, CD45RB, CD45RO and several others.The RA3-6B2 antibody is one of the most consistently used leukocyte markers for B cells, T cell subsets and NK cell subsets in human and mouse. This antibody has also been reported as cross-reactive with feline CD45R/B220.
免疫原
Immunogen information for this antibody is proprietary information.
應用
Research Category
Inflammation & Immunology
Inflammation & Immunology
This rat monoclonal antibody Anti-CD45R (B220) (human/mouse), violetFluor 450, clone RA3-6B2 validated for use in western blotting, IP, IHC, Immunofluorescence & Flow Cytometry for the detection of CD45R (B220).
品質
The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 0.5 µg of this antibody detected CD45R (B220) in one million C57Bl/6 splenocytes.
Flow Cytometry Analysis: 0.5 µg of this antibody detected CD45R (B220) in one million C57Bl/6 splenocytes.
外觀
Size Exclusion Chromatography
Purified Rat Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
儲存和穩定性
It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
法律資訊
violetFluor is a registered trademark of Tonbo Biotechnologies
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
nwg
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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