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Merck
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Key Documents

MABF268

Sigma-Aldrich

Anti-IRF3 Antibody, clone SL-12

clone SL-12, from mouse

别名:

Interferon regulatory factor 3, IRF-3

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

SL-12, monoclonal

物種活性

human, mouse

技術

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

同型

IgG1κ

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... IRF3(3661)

一般說明

Interferon regulatory factors (IRFs) comprise a family of transcription factors that function with the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection. IRF-3 can inhibit cell growth and plays a critical role in controlling the expression of genes in the innate immune response. In unstimulated cells, IRF-3 is present in the cytoplasm. Viral infection results in phosphorylation of IRF-3 and leads to its translocation to the nucleus where it activates promoters containing IRF-3-binding sites. Phosphorylation of IRF-3 occurs at a cluster of C-terminal serine and threonine residues (between 385 and 405) leading to its association with the p300/CBP coactivator protein that promotes DNA binding and transcriptional activity.

免疫原

Recombinant protein corresponding to human IRF3.

應用

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected IRF3 in HeLa, A-431, HepG2, and NIH/3T3 cells. DyLight 488 is a registered trademark of Thermo Fisher Scientific.
Immunoprecipitation Analysis: A representative lot immunoprecipitated IRF3 in HEC1B cells treated or untreated with virus and M059J DNA-PK - and M059J cells with DNA-PK+ (Karpova, A.Y., et al. (2002). PNAS. 99(5):2818-2823).
Western Blotting Analysis: A representative lot detected IRF3 in HFK (human foreskin keratinocytes) cells expressing BPV ORF′s (Ronco, L.V., et al. (1998) Genes Dev. 12:2061-2072).
Western Blotting Analysis: A representative lot detected IRF3 in Transfected Mock and SeV infected 293 cells (Basler, C.F., et al. (2003) Journal of Virology. 77(14):7945-7956).
Immunocytochemistry Analysis: A representative lot detected IRF3 in hec-1b cells infected with delNS1 or PR8 (Talon. J., et al. (2000). Journal of Virology. 74(17):7989-7996).
Immunocytochemistry Analysis: A representative lot detected IRF3 in HEC1B cells treated or untreated with virus (Karpova, A.Y., et al. (2002). PNAS. 99(5):2818-2823).
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This Anti-IRF3 Antibody, clone SL-12 is validated for use in Western Blotting and Immunocytochemistry and Immunoprecipitation for the detection of IRF3.

品質

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected IRF3 in 10 µg of HeLa cell lysate.

標靶描述

~49 kDa observed. IRF3 has isoforms at ~49 kDa, ~47 kDa, ~33 kDa, ~16 kDa, & ~12 kDa

聯結

Replaces: MABF21

外觀

Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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J Talon et al.
Journal of virology, 74(17), 7989-7996 (2000-08-10)
We present a novel mechanism by which viruses may inhibit the alpha/beta interferon (IFN-alpha/beta) cascade. The double-stranded RNA (dsRNA) binding protein NS1 of influenza virus is shown to prevent the potent antiviral interferon response by inhibiting the activation of interferon
Christopher F Basler et al.
Journal of virology, 77(14), 7945-7956 (2003-06-28)
The Ebola virus VP35 protein was previously found to act as an interferon (IFN) antagonist which could complement growth of influenza delNS1 virus, a mutant influenza virus lacking the influenza virus IFN antagonist protein, NS1. The Ebola virus VP35 could
L V Ronco et al.
Genes & development, 12(13), 2061-2072 (1998-07-03)
Interferon regulatory factor-3 (IRF-3) was found to specifically interact with HPV16 E6 in a yeast two-hybrid screen. IRF-3 is activated by the presence of double-stranded RNA or by virus infection to form a stable complex with other transcriptional regulators that
Ning Yang et al.
Nature communications, 13(1), 1671-1671 (2022-03-31)
The pulmonary immune system consists of a network of tissue-resident cells as well as immune cells that are recruited to the lungs during infection and/or inflammation. How these immune components function during an acute poxvirus infection is not well understood.

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