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Merck
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Key Documents

MABF235

Sigma-Aldrich

Anti-AIRE Antibody, clone Mab155

clone Mab155, 1 mg/mL, from mouse

别名:

Autoimmune regulator, Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein, APECED protein

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

Mab155, monoclonal

物種活性

human

濃度

1 mg/mL

技術

flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable

同型

IgG2aκ

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... AIRE(326)

一般說明

AIRE (Autoimmune Regulator) is a human gene that is expressed primarily in the thymus. It causes transcription of a wide selection of organ-specific genes, reducing the threat of autoimmunity occurring by allowing the elimination of autoreactive T cells via negative selection. Defects in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendo-crinopathy-candidiasis-ectodermal dystrophy (APECED). Disruption of AIRE results in the development of a range of autoimmune diseases, the most common clinical conditions in the syndrome are hypoparathyroidism, primary adrenocortical failure and chronic mucocutaneous candidiasis. Aire functions through initiating the transcription of a diverse set of self-antigens, such as insulin, in the thymus. This expression then allows maturing thymocytes to become tolerant towards peripheral organs, thereby suppressing autoimmune disease. Aire is atranscriptional regulator protein that binds to DNA as dimer and tetramer, but not as a monomer. It forms nuclear bodies and interacts with the transcriptional coactivator CBP (CREBBP). At least three splice variant mRNAs products have been described including one which results in a premature stop codon and a transcript predicted to be a candidate for nuclear-mediated decay (NMD).

免疫原

His-tagged recombinant protein corresponding to human AIRE.

應用

Research Category
Inflammation & Immunology
Research Sub Category
Immunological Signaling
This Anti-AIRE Antibody, clone Mab155 is validated for use in Flow Cytometry and Western Blotting and Immunocytochemistry for the detection of AIRE.
Western Blotting Analysis: A representative lot detected AIRE in Cos1 transfected cell lysate (Rinderle, C., et al. (1999). Human Molecular Genetics. 8(2):277-290).
Immunocytochemistry Analysis: A representative lot detected AIRE in freshly isolated endogenous peripheral blood lymphocytes (Rinderle, C., et al. (1999). Human Molecular Genetics. 8(2):277-290).

品質

Evaluated by Flow Cytometry in human PBMC cells.

Flow Cytometry Analysis: 0.5 µg of this antibody detected AIRE in human PBMC cells.

標靶描述

58 kDa calculated

外觀

Protein G Purified
Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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C Rinderle et al.
Human molecular genetics, 8(2), 277-290 (1999-02-05)
The gene responsible for autoimmune polyendocrino-pathy candidiasis ectodermal dystrophy (APECED) recently has been positionally cloned to 21q22.3. This novel gene, AIRE, encodes for a predicted 57.7 kDa protein featuring two PHD-type zinc fingers shared by other proteins involved in chromatin-mediated

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