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Merck
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Key Documents

MABF147D

Sigma-Aldrich

Anti-Ly-6G (mouse), APC, clone RB6-8C5 Antibody

clone RB6-8C5, from rat, Allophycocyanin conjugate

别名:

Lymphocyte antigen 6G (Ly-6G), Ly-6G.1, Ly-6G

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rat

品質等級

共軛

Allophycocyanin conjugate

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

RB6-8C5, monoclonal

物種活性

mouse

包裝

antibody small pack of 25 μg

技術

flow cytometry: suitable

同型

IgG2bκ

UniProt登錄號

目標翻譯後修改

unmodified

基因資訊

mouse ... Ly6G(546644)

一般說明

The RB6-8C5 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils. In the mouse, the RB6-8C5 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes.Note: The RB6-8C5 antibody has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399-2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C. Other reports suggest that this antibody is specific for Ly-6G, without cross-reactivity for Ly-6C (Nagendra S. and Schlueter AJ. 2003. Cytometry A, 58(2): 195-200.

應用

This rat monoclonal Anti-Ly-6G Antibody (mouse), APC, clone RB6-8C5 , Cat. No. MABF1477 is validated for use in Flow Cytometry, for the detection of Ly-6G.

品質

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.125 μg from a representative lot detected Ly-6G in one million C57Bl/6 bone marrow cells.

其他說明

Concentration: Please refer to lot specific datasheet.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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