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Merck
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Key Documents

MABF146C

Sigma-Aldrich

Anti-CD45 (Mouse), PE-Cy7, clone 30-F11 Antibody

clone 30-F11, from rat, PE-Cy7

别名:

Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen (L-CA), Lymphocyte antigen 5 (Ly-5), T200, CD45, CD45

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.44

生物源

rat

品質等級

共軛

PE-Cy7

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

30-F11, monoclonal

物種活性

mouse

包裝

antibody small pack of 25 μg

技術

flow cytometry: suitable

同型

IgG2bκ

UniProt登錄號

目標翻譯後修改

unmodified

基因資訊

mouse ... Ptprc(19264)

一般說明

The 30-F11 antibody reacts with mouse CD45, which is one of the most abundant hematopoietic markers and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). CD45 is a protein tyrosine phosphatase existing in several isoforms, each being generated and expressed in cell-specific patterns. With its broad cell distribution, CD45 is critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA, CD45RB, CD45RO and others. The 30-F11 antibody is widely used as a leukocyte marker for B cells, T cell subsets and NK cell subsets.

免疫原

Immunogen information for this antibody is proprietary information.

應用

Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This rat monoclonal Anti-CD45 Antibody (Mouse), PE-Cy7, clone 30-F11, Cat. No. MABF1465 is validated for use in Flow Cytometry, for the detection of CD45.

品質

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.125 μg from a representative lot detected CD45 in one million C57Bl/6 splenocytes.

外觀

Purified by size-exclusion chromatography
Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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