跳转至内容
Merck
所有图片(1)

主要文件

MABE343-AF647

Sigma-Aldrich

抗-嘌呤霉素抗体,克隆12D10,Alexa Fluor 647偶联物

clone 12D10, 0.5 mg/mL, from mouse

别名:

Alexa Fluor 647 Anti-Puromycin, Clone 12D10 Anti-Puromycin, Puromycin Detection Antibody

登录查看公司和协议定价


About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

共軛

ALEXA FLUOR 647

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

12D10, monoclonal

物種活性

human

濃度

0.5 mg/mL

技術

immunocytochemistry: suitable

運輸包裝

wet ice

目標翻譯後修改

unmodified

一般說明

嘌呤霉素是一种氨基核苷酸抗生素,来源于白黑链霉菌,是蛋白合成抑制剂,可通过核糖体中的过早链终止来阻止翻译。抗嘌呤霉素的单克隆抗体可与标准免疫化学方法一起用于直接监测翻译,这种方法称为翻译表面感应(SUnSET)。该分子的一部分类似于氨基酰化tRNA的3′端,这使其可用于蛋白质翻译分析。嘌呤霉素诱导胸腺细胞和HL-60白血病细胞内DNA断裂。
该抗嘌呤霉素(克隆12D10)抗体经过免疫细胞化学应用验证,可用于检测嘌呤霉素。

特異性

与嘌呤霉素预孵育时,预期与所有物种细胞发生反应。

品質

通过免疫细胞化学分析未处理和嘌呤霉素处理的HeLa细胞进行评估。

免疫细胞化学分析:用该抗体的1:2,500稀释液在嘌呤霉素处理的HeLa细胞中检测掺入嘌呤霉素的新合成蛋白。

Alexa Fluor是Life Technologies的注册商标。

標靶描述

请参阅货号MABE343了解观测到的分子量信息。

法律資訊

ALEXA FLUOR is a trademark of Life Technologies

未找到合适的产品?  

试试我们的产品选型工具.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

Hongcheng Cheng et al.
Nature metabolism, 5(2), 314-330 (2023-01-31)
The accumulation of acidic metabolic waste products within the tumor microenvironment inhibits effector functions of tumor-infiltrating lymphocytes (TILs). However, it remains unclear how an acidic environment affects T cell metabolism and differentiation. Here we show that prolonged exposure to acid
Noga Ron-Harel et al.
Cell reports, 28(12), 3011-3021 (2019-09-19)
T cell stimulation is metabolically demanding. To exit quiescence, T cells rely on environmental nutrients, including glucose and the amino acids glutamine, leucine, serine, and arginine. The expression of transporters for these nutrients is tightly regulated and required for T cell activation.
Seema Qamar et al.
Cell, 173(3), 720-734 (2018-04-21)
Reversible phase separation underpins the role of FUS in ribonucleoprotein granules and other membrane-free organelles and is, in part, driven by the intrinsically disordered low-complexity (LC) domain of FUS. Here, we report that cooperative cation-π interactions between tyrosines in the
Noémie Mazaré et al.
Cell reports, 32(8), 108076-108076 (2020-08-28)
Local translation is a conserved mechanism conferring cells the ability to quickly respond to local stimuli. In the brain, it has been recently reported in astrocytes, whose fine processes contact blood vessels and synapses. Yet the specificity and regulation of
Eric J Allenspach et al.
The Journal of experimental medicine, 218(5) (2021-03-17)
SAMD9L is an interferon-induced tumor suppressor implicated in a spectrum of multisystem disorders, including risk for myeloid malignancies and immune deficiency. We identified a heterozygous de novo frameshift variant in SAMD9L in an infant with B cell aplasia and clinical

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门