推荐产品
生物源
mouse
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
N69, monoclonal
物種活性
mouse, rat, bovine, human
技術
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
同型
IgG1κ
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... HSPA8(3312)
一般說明
Heat shock cognate 71 kDa protein (UniProt P19120; also known as Heat shock 70 kDa protein 8, HSC70, HSC71, HSC73) is encoded by the HSPA8 (also known as HSC70, HSC73) gene (Gene ID 281831) in bovine species. The mammalian HSP70 family consists of the mitochondrial HSP75, the endoplasmic HSP78, the cytoplasmic HSC70 (a.k.a. HSC73 and HSP73) and its inducible counterpart HSP72 (a.k.a. HSP70). The latter two have similar structures and properties, and both of them bind misfolded, newly synthesized polypeptides in an ATP-dependent manner, reflecting their chaperonic activity. All cells constitutively express HSC70. On the other hand, the expression of HSP72 in normal cells is mainly stress inducible, although constitutively high HSP72 expressions are observed in malignant human tumors of various origins. The induced HSP72 expression enables stressed cells to better cope with the elevation of unfolded/denatured proteins and prevent apoptosis under various stress conditions.
特異性
Clone N69 reacts with the constitutively expressed HSPA8/Hsc70 (HSC73), but not the heat schock-inducible HSP72 (Demidov, O.N., et al. (1999). Eur. J. Cardiothorac. Surg. 16(4):444-449).
免疫原
Co-purified Hsc73 and Hsp72 proteins from bovine skeletal muscle.
應用
Anti- HSPA8/Hsc70 Antibody, clone N69 is an antibody against HSPA8/Hsc70 for use in Western Blotting, Immunocytochemistry, Flow Cytometry, Immunohistochemistry.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
Nuclear Receptors
Western Blotting Analysis: 2.0 µg/mL of this antibody detected HSPA8/Hsc70 in 10 µg rat C6 glioma, human T47D ductal breast epithelial tumor, and human K-562 erythroleukemia cell lysates.
Immunocytochemistry Analysis: 2.0 µg/mL of a representative lot detected the constitutive HSPA8/Hsc70 expression in C6 rat glioma, B16 mouse melanoma, and K-562 human erythroblastoma cells (Courtesy of Dr. Irina V. Guzhova and Dr. Boris Margulis, Russian Academy of Sciences).
Flow Cytometry Analysis: 2.0 µg/mL of a representative lot detected the constitutive HSPA8/Hsc70 expression in C6 rat glioma, B16 mouse melanoma, and K-562 human erythroblastoma cells (Courtesy of Dr. Irina V. Guzhova and Dr. Boris Margulis, Russian Academy of Sciences).
Immunohistochemistry Analysis: A representative lot detected bovine HSPA8/Hsc70 in anterior hypothalamus frozen sections from rats received purified bovine Hsc70 and Hsp72 proteins via intracerebroventricular (i.c.v.) microinjections (Ekimova, I.V., et al. (2010). J. Neurochem. 115(4):1035-1044).
Western Blotting Analysis: A representative lot detected HSPA8/Hsc70 expression in MCF7 cells (Hoyer-Hansen, M., et al. (2005). Cell Death Differ. 12(10):1297-1309).
Western Blotting Analysis: A representative lot detected HSPA8/Hsc70 expression in human breast cancer cell lines (MDA-MB-468, MCF-7, BT-549, and SK-BR-3), non-tumorigenic breast-derived HBL-100 epithelial cells, and WI-38 normal lung fibroblasts (Nylandsted, J., et al. (2000). Proc. Natl. Acad. Sci. U. S. A. 97(14):7871-7876).
Western Blotting Analysis: A representative lot detected HSPA8/Hsc70 (HSC73) purified from bovine skeletal muscle and human myocardium, but not the co-purified HSP72 (Demidov, O.N., et al. (1999). Eur. J. Cardiothorac. Surg. 16(4):444-449).
Immunocytochemistry Analysis: 2.0 µg/mL of a representative lot detected the constitutive HSPA8/Hsc70 expression in C6 rat glioma, B16 mouse melanoma, and K-562 human erythroblastoma cells (Courtesy of Dr. Irina V. Guzhova and Dr. Boris Margulis, Russian Academy of Sciences).
Flow Cytometry Analysis: 2.0 µg/mL of a representative lot detected the constitutive HSPA8/Hsc70 expression in C6 rat glioma, B16 mouse melanoma, and K-562 human erythroblastoma cells (Courtesy of Dr. Irina V. Guzhova and Dr. Boris Margulis, Russian Academy of Sciences).
Immunohistochemistry Analysis: A representative lot detected bovine HSPA8/Hsc70 in anterior hypothalamus frozen sections from rats received purified bovine Hsc70 and Hsp72 proteins via intracerebroventricular (i.c.v.) microinjections (Ekimova, I.V., et al. (2010). J. Neurochem. 115(4):1035-1044).
Western Blotting Analysis: A representative lot detected HSPA8/Hsc70 expression in MCF7 cells (Hoyer-Hansen, M., et al. (2005). Cell Death Differ. 12(10):1297-1309).
Western Blotting Analysis: A representative lot detected HSPA8/Hsc70 expression in human breast cancer cell lines (MDA-MB-468, MCF-7, BT-549, and SK-BR-3), non-tumorigenic breast-derived HBL-100 epithelial cells, and WI-38 normal lung fibroblasts (Nylandsted, J., et al. (2000). Proc. Natl. Acad. Sci. U. S. A. 97(14):7871-7876).
Western Blotting Analysis: A representative lot detected HSPA8/Hsc70 (HSC73) purified from bovine skeletal muscle and human myocardium, but not the co-purified HSP72 (Demidov, O.N., et al. (1999). Eur. J. Cardiothorac. Surg. 16(4):444-449).
品質
Evaluated by Western Blotting in MCF7 cell lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected HSPA8/Hsc70 in 10 µg MCF7 cell lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected HSPA8/Hsc70 in 10 µg MCF7 cell lysate.
標靶描述
~71 kDa observed
外觀
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at 2-8°C from date of receipt.
其他說明
Concentration: Please refer to lot specific datasheet.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Vitamin D analog EB1089 triggers dramatic lysosomal changes and Beclin 1-mediated autophagic cell death.
H?yer-Hansen, M; Bastholm, L; Mathiasen, IS; Elling, F; Jaattela, M
Cell Death and Differentiation null
Attila Simor et al.
Molecular and cellular neurosciences, 79, 64-80 (2017-01-15)
Acute total sleep deprivation (SD) impairs memory consolidation, attention, working memory and perception. Structural, electrophysiological and molecular experimental approaches provided evidences for the involvement of sleep in synaptic functions. Despite the wide scientific interest on the effects of sleep on
J Nylandsted et al.
Proceedings of the National Academy of Sciences of the United States of America, 97(14), 7871-7876 (2000-07-08)
Heat shock protein 70 is an antiapoptotic chaperone protein highly expressed in human breast tumors and tumor cell lines. Here, we demonstrate that the mere inhibition of its synthesis by adenoviral transfer or classical transfection of antisense Hsp70 cDNA (asHsp70)
Masaharu Somiya et al.
Journal of extracellular vesicles, 7(1), 1440132-1440132 (2018-03-08)
Extracellular vesicles (EVs) deliver biologically active cargos from donor cells to recipient cells for intercellular communication. Since the existence of RNA cargo was discovered, EVs have been considered to be useful drug-delivery systems. Specifically, EVs from bovine milk (mEV) are
Heat shock proteins in cardiosurgery patients.
Demidov, ON; Tyrenko, VV; Svistov, AS; Komarova, YY; Karpishenko, AI; Margulis, BA; Shevchenko, YL
European Journal of Cardio-Thoracic Surgery : Official Journal of the European Association For Cardio-Thoracic Surgery null
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