MABC541

Anti-Cullin-3 Antibody, clone 3A8.1

clone 3A8.1, from mouse

• 别名: Transient receptor potential cation channel subfamily V member 4, TrpV4, Osm-9-like TRP channel 4, OTRPC4, Transient receptor potential protein 12, TRP12, Vanilloid receptor-like channel 2, Vanilloid receptor-like protein 2, VRL-2, Vanilloid receptor-rel
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 3A8.1, monoclonal
• 物種活性: monkey, mouse
• 物種活性（以同源性預測）: human (based on 100% sequence homology)
• 技術: western blot: suitable
• 同型: IgG1κ
• NCBI登錄號: NP_003581
• UniProt登錄號: Q13618
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... CUL3(8452)

说明

一般說明

Cullin-3 (UniProt Q13618) is encoded by the CUL3 (also known as PHA2E, KIAA0617) gene (Gene ID 8452) in human. Polyubiquitination of proteins is mediated by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). There exist RING finger-type, HECT-type, and U-box type of E3 ligases. Cullin-3 belongs to the cullin family of hydrophobic proteins that confer substrate specificity to the multimeric Cullin-RING E3 ubiquitin ligase (CRL) complexes by acting as scaffold proteins. The activity of CRLs is regulated by Cullin Neddylation via the reversible conjugation of a small ubiquitin-type protein known as Neural precursor cell expressed developmentally down-regulated protein 8 (Nedd8). Cullin-3-based CRLs rely on Cullin-3 interaction with bric-a-brac/tramtrack/broad-complex (BTB) domain-containing proteins for targets recruitment.

免疫原

Epitope: C-terminal

GST-tagged recombinant protein corresponding to the C-terminal of human Cullin-3.

應用

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-Cullin-3 Antibody, clone 3A8.1 is validated for use in Western Blotting for the detection of Cullin-3.

品質

Evaluated by Western Blotting in mouse neuroblastoma Neuro-2a (N2a) cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Cullin-3 in 10 µg of mouse neuroblastoma Neuro-2a (N2a) cell lysate.

標靶描述

~88 kDa observed. Uncharacterized band(s) may appear in some lysates.

外觀

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable