跳转至内容
Merck
所有图片(1)

主要文件

MABC1791

Sigma-Aldrich

Anti-hMENA11a Antibody, clone A1

别名:

ENAH, Protein enabled homolog, hMENA11a, Mammalian enabled variant 11a

登录查看公司和协议定价


About This Item

分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

mouse

品質等級

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

A1, monoclonal

分子量

calculated mol wt 66.5 kDa
observed mol wt ~90 kDa

純化經由

using protein G

物種活性

human

包裝

antibody small pack of 100

技術

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

同型

IgG1κ

表位序列

C-terminal

Protein ID登錄號

UniProt登錄號

儲存溫度

2-8°C

基因資訊

human ... ENAH(55740)

特異性

Clone A1 is a mouse monoclonal antibody that detects hMENA 11a. It targets an epitope within 18 amino acids from the C-terminal region.

免疫原

A linear peptide corresponding to 18 amino acids from the C-terminal region of human MENA protein belonging to 11a exon.

應用

Quality Control Testing

Evaluated by Western Blotting in T47D cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected hMENA11a in T47D cell lysate.

Tested Applications

Immunocytochemistry Analysis: A representative lot detected MENA11a/ENAH in Western Blotting application (Di Modugno, F., et al. (2012). Proc Natl Acad Sci USA. 109(47):19280-5; Melchionna, R., et al. (2020). EMBO Rep. 21(11):e50078).

Western Blotting Analysis: A representative lot detected MENA11a/ENAH in Western Blotting applications (Di Modugno, F., et al. (2012). Proc Natl Acad Sci USA. 109(47):19280-5).

Immunohistochemistry Applications: A representative lot detected MENA11a/ENAH in Immunohistochemistry applications (Di Modugno, F., et al. (2012). Proc Natl Acad Sci USA. 109(47):19280-5).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

標靶描述

Protein enabled homolog (UniProt: A0A097PIC4; also known as hMENA, Mammalian enabled variant 11a, ENAH) is encoded by the ENAH (also known as MENA) gene (Gene ID: 55740) in human. hMENA is a homotetrameric actin regulatory protein of the ENA/VASP family that plays a key role in cell processes that rely on actin cytoskeleton dynamics. Its expression is observed in a variety of tissues. It is overexpressed in a majority of breast cancer cell lines and primary breast tumor lesions. Its EVH2 domain mediates tetramerization, F-actin binding, and actin bundle formation. The EVH2 domain is comprised of three regions: Block A (aa 391-411) is a thymosin-like domain that is essential for G-actin binding; block B (aa 442-459) is required for F-actin binding and subcellular location, and block C (aa 554-588) is required for tetramerization. It also contains a KLKR motif (aa 400-403) within block A that is reported to be essential for G-actin binding and actin polymerization. hMENA is absent in normal breast tissue, but it is overexpressed in breast tumors, where its expression correlates with HER-2-positive, ER-negative, and high Ki 67 phenotype. Depletion of hMENA/ hMENA11 is reported to decrease HER3 phosphorylation, inhibits EGF- and NRG-mediated activation of EGFR and HER-2 and counteract growth factor-mediated cell proliferation. Silencing of hMENA/hMENA11a is reported to switch on molecules linked to apoptosis, including cleaved forms of poly (ADP-ribose) polymerase and caspase 9. A hMENA v6 variant of hMENA has also been described that increases cell invasion. In an isogenic model of human breast cancer progression, hMENA11a expression is observed in premalignant cells, whereas hMENA v6 expression is restricted to invasive cancer cells. (Ref.: Melchionna, R., et al. (2020). EMBO Rep. 21; e50078; Trono, P., et al. (2016). Mol. Cell. Oncol. 3(2); e1083648; Di Modugnoa, F., et al. (2012). Proc. Natl. Acad. Sci. USA. 109(47); 19280-19285).

外觀

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

重構

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommended storage: +2°C to +8°C.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

未找到合适的产品?  

试试我们的产品选型工具.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门