生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
A1, monoclonal
分子量
calculated mol wt 66.5 kDa
observed mol wt ~90 kDa
純化經由
using protein G
物種活性
human
包裝
antibody small pack of 100
技術
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
同型
IgG1κ
表位序列
C-terminal
Protein ID登錄號
UniProt登錄號
儲存溫度
2-8°C
基因資訊
human ... ENAH(55740)
特異性
Clone A1 is a mouse monoclonal antibody that detects hMENA 11a. It targets an epitope within 18 amino acids from the C-terminal region.
免疫原
A linear peptide corresponding to 18 amino acids from the C-terminal region of human MENA protein belonging to 11a exon.
應用
Quality Control Testing
Evaluated by Western Blotting in T47D cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected hMENA11a in T47D cell lysate.
Tested Applications
Immunocytochemistry Analysis: A representative lot detected MENA11a/ENAH in Western Blotting application (Di Modugno, F., et al. (2012). Proc Natl Acad Sci USA. 109(47):19280-5; Melchionna, R., et al. (2020). EMBO Rep. 21(11):e50078).
Western Blotting Analysis: A representative lot detected MENA11a/ENAH in Western Blotting applications (Di Modugno, F., et al. (2012). Proc Natl Acad Sci USA. 109(47):19280-5).
Immunohistochemistry Applications: A representative lot detected MENA11a/ENAH in Immunohistochemistry applications (Di Modugno, F., et al. (2012). Proc Natl Acad Sci USA. 109(47):19280-5).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in T47D cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected hMENA11a in T47D cell lysate.
Tested Applications
Immunocytochemistry Analysis: A representative lot detected MENA11a/ENAH in Western Blotting application (Di Modugno, F., et al. (2012). Proc Natl Acad Sci USA. 109(47):19280-5; Melchionna, R., et al. (2020). EMBO Rep. 21(11):e50078).
Western Blotting Analysis: A representative lot detected MENA11a/ENAH in Western Blotting applications (Di Modugno, F., et al. (2012). Proc Natl Acad Sci USA. 109(47):19280-5).
Immunohistochemistry Applications: A representative lot detected MENA11a/ENAH in Immunohistochemistry applications (Di Modugno, F., et al. (2012). Proc Natl Acad Sci USA. 109(47):19280-5).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
標靶描述
Protein enabled homolog (UniProt: A0A097PIC4; also known as hMENA, Mammalian enabled variant 11a, ENAH) is encoded by the ENAH (also known as MENA) gene (Gene ID: 55740) in human. hMENA is a homotetrameric actin regulatory protein of the ENA/VASP family that plays a key role in cell processes that rely on actin cytoskeleton dynamics. Its expression is observed in a variety of tissues. It is overexpressed in a majority of breast cancer cell lines and primary breast tumor lesions. Its EVH2 domain mediates tetramerization, F-actin binding, and actin bundle formation. The EVH2 domain is comprised of three regions: Block A (aa 391-411) is a thymosin-like domain that is essential for G-actin binding; block B (aa 442-459) is required for F-actin binding and subcellular location, and block C (aa 554-588) is required for tetramerization. It also contains a KLKR motif (aa 400-403) within block A that is reported to be essential for G-actin binding and actin polymerization. hMENA is absent in normal breast tissue, but it is overexpressed in breast tumors, where its expression correlates with HER-2-positive, ER-negative, and high Ki 67 phenotype. Depletion of hMENA/ hMENA11 is reported to decrease HER3 phosphorylation, inhibits EGF- and NRG-mediated activation of EGFR and HER-2 and counteract growth factor-mediated cell proliferation. Silencing of hMENA/hMENA11a is reported to switch on molecules linked to apoptosis, including cleaved forms of poly (ADP-ribose) polymerase and caspase 9. A hMENA v6 variant of hMENA has also been described that increases cell invasion. In an isogenic model of human breast cancer progression, hMENA11a expression is observed in premalignant cells, whereas hMENA v6 expression is restricted to invasive cancer cells. (Ref.: Melchionna, R., et al. (2020). EMBO Rep. 21; e50078; Trono, P., et al. (2016). Mol. Cell. Oncol. 3(2); e1083648; Di Modugnoa, F., et al. (2012). Proc. Natl. Acad. Sci. USA. 109(47); 19280-19285).
外觀
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
重構
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommended storage: +2°C to +8°C.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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