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Merck
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Key Documents

MABC1690H

Sigma-Aldrich

Anti-RAINBOW Antibody, HRP conjugated

from mouse, peroxidase conjugate

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.45

生物源

mouse

品質等級

共軛

peroxidase conjugate

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

monoclonal

物種活性(以同源性預測)

all

包裝

antibody small pack of 25 μg

技術

western blot: suitable

同型

IgG1κ

運輸包裝

ambient

目標翻譯後修改

unmodified

一般說明

Pre-stained proteins are commonly used as molecular weight markers in electrophoresis. Several protein markers are available as pre-stained proteins with vinyl sulfone dyes. After separation through electrophoresis, the protein of interest is visualized by specific antibody coupled to a fluorophore or by chemiluminescence detection system. Anti-RAINBOW antibody is a unique antibody that can detect pre-stained molecular markers from different vendors without the need to perform a manual marking and estimating molecular weights. Anti-RAINBOW antibody detects all marker proteins in commercially available RAINBOW markers tested and lacks cross-reactivity with unstained proteins in whole cell lysates of a variety of species. It displays equal detection of Remazol-dye stained proteins: phosphorylase b (red), BSA and ADH (blue), chymotrypsin (yellow) and lysozyme (orange).

特異性

Anti-RAINBOW, HRP-conjugated is a mouse monoclonal antibody that can be used as a universal tool for visualizing prestained protein molecular weight markers in Western blot analysis. It recognizes and cross-reacts with comparable affinity with four different Remazol dye immunogens.

免疫原

Purified Remazol Brilliant Blue R (RBBR)-BSA, followed by RO16-Chymotrypsin, RGY-Catalase and RF3B-Lysozyme.

應用

Anti-RAINBOW, HRP-conjugated, Cat. No. MABC1690H, is a mouse monoclonal antibody that recognizes four different Remazol dye-conjugated proteins and can be used to visualize prestained molecular weight markers in Western Blotting.
Research Category
Secondary & Control Antibodies
Western Blotting Analysis: A representative lot detected Remzaol-dye prestained protein standards in Western Blotting applications (Schuchner, S., et. al. (2016). Sci Rep. 6:31363).

Western Blotting Analysis: A 1:500 and 1:1,000 dilution from a representative lot detected protein standard.

品質

Evaluated by Western Blotting in Blue Pre-Stained Protein Standard.

Western Blotting Analysis: A 1:10,000; 1:20,000; 1:40,000; 1:80,000 dilution of this antibody detected Remzaol-dye prestained protein standards.

外觀

Protein G purified
Purified mouse monoclonal antibody IgG1 in PBS with StabilZyme® HRP Conjugate Stabilizer and 0.05% Kathon.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

法律資訊

StabilZyme is a registered trademark of SurModics, Inc.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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象形圖

Exclamation markEnvironment

訊號詞

Warning

危險聲明

閃點(°F)

does not flash

閃點(°C)

does not flash


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