推荐产品
生物源
mouse
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
SMP14, monoclonal
物種活性
rat, human, mouse
製造商／商標名
Chemicon®
技術
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
同型
IgG1
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... MDM2(4193)
mouse ... Mdm2(17246)
rat ... Mdm2(314856)
一般說明
MDM2 is over-expressed in many tumors. Its principal function is the ubiquitination and degradation of p53 tumour suppressor protein. This monoclonal antibody also recognizes a peptide epitope around Thr-216 of murine MDM2 when Thr-216 is unphosphorylated (Zhang & Prives, 2001). SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.
特異性
Recognizes human MDM2.
免疫原
Synthetic peptide corresponding to residues 154-167 (CSRPSTSSRRRAISE) of human MDM2 cross-linked to KLH using glutaldehyde.
應用
Research Sub Category
Ubiquitin & Ubiquitin Metabolism
RNA Binding Protein (RBP)
Ubiquitin & Ubiquitin Metabolism
RNA Binding Protein (RBP)
Use Anti-MDM2 Antibody, clone SMP14 (Mouse Monoclonal Antibody) validated in IP, IHC, IHC(P) to detect MDM2 also known as Murine Double Minute 2.
Western blot
Immunohistochemistry (Frozen and Paraffin-embedded sections)
Immunoprecipitation
ELISA
Optimal working dilutions must be determined by end user.
Immunohistochemistry (Frozen and Paraffin-embedded sections)
Immunoprecipitation
ELISA
Optimal working dilutions must be determined by end user.
外觀
Format: Purified
Liquid in 0.02M phosphate buffer, 0.25M NaCl, pH 7.6, with 0.1% sodium azide
分析報告
Control
POSITIVE CONTROL: Breast carcinoma
POSITIVE CONTROL: Breast carcinoma
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Cyclin a-CDK phosphorylation regulates MDM2 protein interactions.
Zhang, T and Prives, C
The Journal of Biological Chemistry, 276, 29702-29710 (2001)
Richard O Adeyemi et al.
Journal of virology, 86(15), 8328-8332 (2012-05-25)
The DNA damage response to infection with minute virus of mice (MVM) leads to activated p53; however, p21 levels are reduced via a proteasome-mediated mechanism. This loss was sustained, as virus replicated in infected cells held at the G(2)/M border.
Gengze Wu et al.
Circulation, 130(17), 1452-1465 (2014-08-27)
Long noncoding RNAs (lncRNAs) have recently been implicated in many biological processes and diseases. Atherosclerosis is a major risk factor for cardiovascular disease. However, the functional role of lncRNAs in atherosclerosis is largely unknown. We identified lincRNA-p21 as a key
S M Picksley et al.
Oncogene, 9(9), 2523-2529 (1994-09-01)
The function of p53 is modulated by binding to a number of cellular and viral proteins, such as MDM2 and SV40 large T antigen. An initial immunochemical characterization of the p53-MDM2 complex in a rat fibroblast cell line (Clone 6)
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