推荐产品
生物源
rat
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
AL-2, monoclonal
物種活性
mouse
製造商／商標名
Chemicon®
技術
ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
同型
IgG1κ
NCBI登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
mouse ... Lama1(16772)
一般說明
Laminins are secreted glycoproteins incorporated into cell-associated extracellular matrices and an integral part of the structural scaffolding in most animal tissues. The laminin protein is made up of three polypeptide chains; α, β and γ, accounting for its flexibility in connecting various molecules. When originally characterized, laminin-1 clone AL-2 reacted with Engelbreth-Holm-Swarm (EHS) mouse laminin A & B chains on the long arm near the cross region, (Skubitz, 1987). More recently, investigations, (Schèele, 2006) have shown that the clone binds laminin β1 under reducing conditions as well as native ELISAs and that the affinity is increased by the presence of the laminin α1 chain. Thus there is significant laminin β1 reactivity with clone AL-2.
特異性
Other species have not been tested.
Reacts with EHS mouse laminin A & B chains on the long arm near the cross region.
免疫原
Epitope: Near cross region
Laminin isolated from EHS mouse sarcoma.
應用
Detect Laminin-1 A&B chains using this Anti-Laminin-1 A&B chains Antibody, cross region, clone AL-2 validated for use in ELISA, IF, IH(P), IP & WB.
Immunofluorescence:
An independent laboratory (Schèele, S., 2006) has verified anti-laminin clone AL-2 to work using immunofluorescence techniques on mouse muscle tissue, including both transgenic and wild-type tissues.
An independent laboratory (Schèele, S., 2006) has verified anti-laminin clone AL-2 to work using immunofluorescence techniques on mouse muscle tissue, including both transgenic and wild-type tissues.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
品質
Evaluated on a representative lot by Western blot on purified mouse laminin-1.
標靶描述
~200 kDa
外觀
Protein G Purified
Format: Purified
Purified in 0.1M Tris-Glycine (pH 7.4) 150mM NaCl with 0.05% NaN3.
儲存和穩定性
Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Laminin isoforms 8 and 10 are primary components of the subendothelial basement membrane promoting interaction with neoplastic lymphocytes.
P Spessotto, Z Yin, G Magro, R Deutzmann, A Chiu, A Colombatti, R Perris
Cancer Research null
Clinical and histopathological analysis of healing process of intraoral reconstruction with ex vivo produced oral mucosa equivalent.
Takeshi Hotta,Satoshi Yokoo,Hiroto Terashi,Takahide Komori
Kobe J. Med. Sci. null
George S Karagiannis et al.
Molecular oncology, 8(2), 178-195 (2013-11-26)
It has been suggested that cancer-associated fibroblasts (CAFs) positioned at the desmoplastic areas of various types of cancer are capable of executing a migratory program, characterized by accelerated motility and collective configuration. Since CAFs are reprogrammed derivatives of normal progenitors
Localization of three distinct heparin-binding domains of laminin by monoclonal antibodies.
Skubitz, A P, et al.
The Journal of Biological Chemistry, 263, 4861-4868 (1988)
Susanne Schéele et al.
Matrix biology : journal of the International Society for Matrix Biology, 25(5), 301-305 (2006-04-25)
We analyzed the reactivity of three different commercially available rat monoclonal antibodies raised against mouse laminin-alpha1beta1gamma1 (laminin-111), AL-1, AL-2, and AL-4. Using ELISA assays, Western blot analysis and immunostainings we present refined epitope maps for these three laminin monoclonals. AL-1
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