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生物源
mouse
品質等級
共軛
FITC conjugate
抗體表格
purified immunoglobulin
無性繁殖
FMC-7, monoclonal
物種活性
human
製造商／商標名
Chemicon®
技術
flow cytometry: suitable
同型
IgM
運輸包裝
wet ice
目標翻譯後修改
unmodified
一般說明
B cells are lymphocytes that play a large role in the humoral immune response as opposed to the cell-mediated immune response that is governed by T cells. The principal function of B cells is to make antibodies against soluble antigens. B cells are an essential component of the adaptive immune system. FMC-7 B-Cell Lymphocyte Marker is a 105 kDa B cell restricted antigen which is expressed on about 50% of adult human peripheral blood B cells. Upon in vivo B cell activation FMC7 expression initially increases. B cells involved in antibody secretion have lost the FMC7 determinant. The monoclonal antibody FMC7 delineates a subpopulation of B lymphocytes in normal blood. Expression of the antigen recognized by FMC7 appears to be maturation-linked, and it serves to distinguish different types of B cell leukemia.
BIOCHEMISTRY: p I = 7.4
特異性
The FMC-7 antibody was not clustered at the 5th Leucocyte Typing Workshop held in Boston USA 1993. This antibody detects a glycoprotein of 105kD found on circulating B-lymphocytes (Brooks et al., 1981). Studies on normal lymphocytes, leukaemic cells and cell lines indicate that FMC-7 is a marker for a limited segment of the B cell maturation pathway (Catovsky et al., 1981; Zola et al., 1984). This glycoprotein is expressed in varying degrees on normal circulating B-cells, chronic B-cell leukaemia (B-CLL), prolymphocytic leukaemia (PLL) and other B cell neoplasias (Zola et al., 1984, 1987; Bloem et al., 1988; Melo et al., 1988; Ferro & Zola, 1990; Mulligan, 1990; Zola et al., 1991; Burthem & Cawley, 1994; Huh et al., 1994). It labels the same population as CD22 antibodies but reacts with a different antigen (Zola et al. 1987).
Cell reactivity:1
Stains peripheral blood B lymphocytes and tonsil B lymphocytes. No reaction with granulocytes, monocytes, platelets, erythrocytes, T lymphocytes or null cells. Reacts with HRIK and Raji cell lines.
Clinical9,12,13 Expression
B cell prolymphocytic leukemia (B-PLL) Strongly positive
Hairy cell leukemia (HCL) Strongly positive
Hairy cell leukaemia variant (HCL-V) Strongly positive
Splenic lymphoma with villous lymphocytes (SLVL) Positive
B cell chronic lymphocytic leukaemia (B-CLL) Negative to weakly positive
Cell reactivity:1
Stains peripheral blood B lymphocytes and tonsil B lymphocytes. No reaction with granulocytes, monocytes, platelets, erythrocytes, T lymphocytes or null cells. Reacts with HRIK and Raji cell lines.
Clinical9,12,13 Expression
B cell prolymphocytic leukemia (B-PLL) Strongly positive
Hairy cell leukemia (HCL) Strongly positive
Hairy cell leukaemia variant (HCL-V) Strongly positive
Splenic lymphoma with villous lymphocytes (SLVL) Positive
B cell chronic lymphocytic leukaemia (B-CLL) Negative to weakly positive
免疫原
The human B-lymphoblastoid cell line, HRIK.
應用
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Immunoglobulins & Immunology
This Anti-FMC-7 B-Cell Lymphocyte Marker Antibody, clone FMC-7, FITC conjugated is validated for use in Flow Cytometry (FC) for the detection of FMC-7 B-Cell Lymphocyte Marker.
This antibody can be used for the differentiation of PLL from B-CLL. It is also useful in confirmation of the diagnosis of other disorders such as HCL, HCL-V and SLVL. Useful for the detection of residual disease in patients undergoing treatment for these disorders.
SUGGESTED USAGE:
Flow cytometry - use 10 μl direct from the vial per 100 μl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.
SUGGESTED USAGE:
Flow cytometry - use 10 μl direct from the vial per 100 μl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.
標靶描述
105 kDa
聯結
Replaces: CBL1416F
外觀
The antibody is supplied in 1.0 ml 0.1M Tris-HCl, 0.5M NaCl, 10mM glycine, pH 8.0, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis and flow cytometry.
儲存和穩定性
Store at 2 to 8°C, for up to 6 months. DO NOT FREEZE. Protect from light.
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
分析報告
Control
Human peripheral blood lymphocytes, HRIK and Raji cell lines
Human peripheral blood lymphocytes, HRIK and Raji cell lines
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
nwg
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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